Deciphering the assembly of enveloped viruses using model lipid membranes

E Brémaud, C Favard, D Muriaux - Membranes, 2022 - mdpi.com
The cell plasma membrane is mainly composed of phospholipids, cholesterol and
embedded proteins, presenting a complex interface with the environment. It maintains a …

Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED–FCS

E Sezgin, F Schneider, S Galiani, I Urbančič… - Nature protocols, 2019 - nature.com
Super-resolution microscopy techniques enable optical imaging in live cells with
unprecedented spatial resolution. They unfortunately lack the temporal resolution required …

HIV-1 Gag specifically restricts PI (4, 5) P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly

C Favard, J Chojnacki, P Merida, N Yandrapalli… - Science …, 2019 - science.org
HIV-1 Gag protein assembles at the plasma membrane of infected cells for viral particle
formation. Gag targets lipids, mainly PI (4, 5) P2, at the inner leaflet of this membrane. Here …

Elucidating carbohydrate-protein interactions using nanoparticle-based approaches

D Kim, N Rahhal, C Rademacher - Frontiers in Chemistry, 2021 - frontiersin.org
Carbohydrates are present on every living cell and coordinate important processes such as
self/non-self discrimination. They are amongst the first molecular determinants to be …

Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes

P Carravilla, A Dasgupta, G Zhurgenbayeva… - Biophysical Reports, 2021 - cell.com
Understanding the plasma membrane nanoscale organization and dynamics in living cells
requires microscopy techniques with high spatial and temporal resolution that permit for long …

Investigation of the Electrokinetic Properties of HIV-Based Virus-Like Particles

T Wolf, C Grau, JF Rosengarten, J Stitz, J Wilkens… - Langmuir, 2024 - ACS Publications
The antigen density on the surface of HIV-based virus-like particles (VLPs) plays a crucial
role in the improvement of HIV vaccine potency. HIV VLPs consist of a dense protein core …

Disruption of Transmembrane Phosphatidylserine Asymmetry by HIV-1 Incorporated SERINC5 Is Not Responsible for Virus Restriction

G Raghunath, EH Abbott, M Marin, H Wu… - Biomolecules, 2024 - mdpi.com
Host restriction factor SERINC5 (SER5) incorporates into the HIV-1 membrane and inhibits
infectivity by a poorly understood mechanism. Recently, SER5 was found to exhibit …

Fluorescence microscopy of the HIV-1 envelope

P Carravilla, JL Nieva, C Eggeling - Viruses, 2020 - mdpi.com
Human immunodeficiency virus (HIV) infection constitutes a major health and social issue
worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane …

Diffusion Measurements at the Nanoscale with STED-FCS

F Schneider, E Sezgin - Fluorescence Spectroscopy and Microscopy in …, 2022 - Springer
The cellular plasma membrane, a seemingly chaotic structure composed of thousands of
lipids and proteins, empowers cellular life and represents a versatile hub for signalling …

Membrane dynamics in health and disease: impact on cellular signalling

P Adhyapak, S Kapoor - The Journal of Membrane Biology, 2019 - Springer
Biological membranes display a staggering complexity of lipids and proteins orchestrating
cellular functions. Superior analytical tools coupled with numerous functional cellular …