Cytokine concentrations measured by multiplex assays in canine peripheral blood samples

KR Richter, AN Nasr, AM Mexas - Veterinary pathology, 2018 - journals.sagepub.com
Cytokines are known to play important roles in a wide range of pathologic conditions
spanning all organ systems in every species studied. As our knowledge of the physiology of …

Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays

HE Lynch, AM Sanchez, MP D'Souza… - Journal of …, 2014 - Elsevier
Luminex bead array assays are widely used for rapid biomarker quantification due to the
ability to measure up to 100 unique analytes in a single well of a 96-well plate. There has …

The statistical value of raw fluorescence signal in Luminex xMAP based multiplex immunoassays

EJ Breen, W Tan, A Khan - Scientific reports, 2016 - nature.com
Tissue samples (plasma, saliva, serum or urine) from 169 patients classified as either
normal or having one of seven possible diseases are analysed across three 96-well plates …

Strengthening the Application of the DAIDS GCLP Guidelines: The Implementation of an Integrated Laboratory Oversight Framework

L Nguyen, A Leach, E Piwowar-Manning… - AIDS Research and …, 2024 - liebertpub.com
The Division of AIDS (DAIDS) Good Clinical Laboratory Practice (GCLP) Guidelines
establish a framework to guide the oversight of laboratories supporting DAIDS-sponsored …

Comparison of ELISA and flow cytometry for measurement of interleukin-1 beta, interleukin-6 and tumor necrosis factor-α

A Çetin, A Şen, I Çetin, B Çimen, L Cimen… - Turkish Journal of …, 2018 - degruyter.com
Background Although majority of the previous studies have shown a good correlation
between enzyme linked immuno sorbent assay (ELISA) and flow cytometry in terms of …

[HTML][HTML] Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays

M Berrong, G Ferrari, C Porth, D Davis, T Denny… - Journal of …, 2023 - Elsevier
The use of conventional serum for supplementation of media in cell-based and single-cell
functional assays has been a major challenge for assay performance, standardization …

Proteomic profiles by multiplex microsphere suspension array

VV Krishnan, SR Selvan, N Parameswaran… - Journal of …, 2018 - Elsevier
Advances in high-throughput proteomic approaches have provided substantial momentum
to novel disease-biomarker discovery research and have augmented the quality of clinical …

The external quality assurance oversight laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay

AM Sanchez, W Rountree, M Berrong, A Garcia… - Journal of …, 2014 - Elsevier
The interferon-gamma enzyme-linked immunospot (IFN-γ ELISpot) assay has been
developed and used as an end-point assay in clinical trials for infectious diseases and …

Performance of International AIDS Vaccine Initiative African clinical research laboratories in standardised ELISpot and peripheral blood mononuclear cell processing …

RK Langat, B Farah, J Indangasi, S Ogola… - African Journal of …, 2021 - scielo.org.za
LANGAT, Robert K. et al. Performance of International AIDS Vaccine Initiative African clinical
research laboratories in standardised ELISpot and peripheral blood mononuclear cell …

Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing

W Rountree, HE Lynch, TN Denny… - Journal of …, 2024 - Elsevier
Bead array assays, such as those sold by Luminex, BD Biosciences, Sartorius, Abcam and
other companies, are a well-established platform for multiplexed quantification of cytokines …