Optical microscopes today have pushed the limits of speed, quality, and observable space in
biological specimens revolutionizing how we view life today. Further, specific labeling of …

The term “in vivo (“in the living”) chemistry” refers to chemical reactions that take place in a
complex living system such as cells, tissue, body liquids, or even in an entire organism. In …

Fluorescence microscopy is essential for studying living cells, tissues and organisms.
However, the fluorescent light that switches on fluorescent molecules also harms the …

Lipid droplets (LDs) play a key role as the hub for lipid metabolism to maintain cellular
metabolic homeostasis. Understanding the functions and changes of LDs in different …

We report on the highly nonlinear behavior observed in the central nervous system tissue of
zebrafish (Danio rerio) when exposed to femtosecond pulses at 1030 nm. At this irradiation …

Light-based photo-stimulation has demonstrated promising effects on stem cell behavior,
particularly in optimizing neurogenesis. However, the precise parameters for achieving …

Zebrafish and organoids, crucial for complex biological studies, necessitate an imaging
system with deep tissue penetration, sample protection from environmental interference, and …

The molecular mechanisms of aging are not fully understood. Here, we used label-free
Stimulated Raman scattering (SRS) microscopy to investigate changes in proteins and lipids …

Phototoxicity in live-cell fluorescence microscopy can compromise experimental outcomes,
yet quantitative methods to assess its impact remain limited. Here we present PhotoFiTT …

We introduce a compact, all-fiber laser system with a gain-managed nonlinear (GMN)
amplified Yb: fiber oscillator and an integrated pulse-picker. The system delivers 39 fs …