Studies on the PII-PipX-NtcA Regulatory Axis of Cyanobacteria Provide Novel Insights into the Advantages and Limitations of Two-Hybrid Systems for Protein …

P Salinas, S Bibak, R Cantos, L Tremiño… - International Journal of …, 2024 - mdpi.com
Yeast two-hybrid approaches, which are based on fusion proteins that must co-localise to
the nucleus to reconstitute the transcriptional activity of GAL4, have greatly contributed to our …

Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe

B Sivakova, A Wagner, M Kretova, J Jakubikova… - Scientific Reports, 2024 - nature.com
In eukaryotes, chromosomal DNA is equally distributed to daughter cells during mitosis,
whereas the number of chromosomes is halved during meiosis. Despite considerable …

Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe

T Selicky, M Jurcik, B Mikolaskova, A Pitelova… - International Journal of …, 2022 - mdpi.com
Pre-mRNA splicing plays a fundamental role in securing protein diversity by generating
multiple transcript isoforms from a single gene. Recently, it has been shown that specific G …

Adenovirus protein VII binds the A-box of HMGB1 to repress interferon responses

EA Arnold, RJ Kaai, K Leung, MR Brinkley… - PLoS …, 2023 - journals.plos.org
Viruses hijack host proteins to promote infection and dampen host defenses. Adenovirus
encodes the multifunctional protein VII that serves both to compact viral genomes inside the …

[HTML][HTML] Tandem affinity purification protocol for isolation of protein complexes from Schizosaccharomyces pombe

L Cipak, T Selicky, J Jurcik, I Cipakova, M Osadska… - STAR protocols, 2022 - Elsevier
Many cellular processes require the activities of complex molecular machines composed of
several protein subunits. Insights into these systems can be gained by isolation of protein …