Imaging flies by fluorescence microscopy: principles, technologies, and applications

S Dunst, P Tomancak - Genetics, 2019 - academic.oup.com
The development of fluorescent labels and powerful imaging technologies in the last two
decades has revolutionized the field of fluorescence microscopy, which is now widely used …

Toward advancing nano-object count metrology: a best practice framework

SC Brown, V Boyko, G Meyers, M Voetz… - Environmental health …, 2013 - ehp.niehs.nih.gov
Background: A movement among international agencies and policy makers to classify
industrial materials by their number content of sub–100-nm particles could have broad …

[HTML][HTML] STED microscopy with optimized labeling density reveals 9-fold arrangement of a centriole protein

L Lau, YL Lee, SJ Sahl, T Stearns, WE Moerner - Biophysical journal, 2012 - cell.com
Super-resolution fluorescence microscopy can achieve resolution beyond the optical
diffraction limit, partially closing the gap between conventional optical imaging and electron …

SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture

M Hilbert, A Noga, D Frey, V Hamel, P Guichard… - Nature cell …, 2016 - nature.com
Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They
are thought to assemble around a nine-fold symmetric cartwheel structure established by …

[HTML][HTML] A genetically-encoded YFP sensor with enhanced chloride sensitivity, photostability and reduced ph interference demonstrates augmented transmembrane …

S Zhong, D Navaratnam, J Santos-Sacchi - PLoS One, 2014 - journals.plos.org
Background Chloride is the major anion in cells, with many diseases arising from disordered
Cl− regulation. For the non-invasive investigation of Cl− flux, YFP-H148Q and its derivatives …

Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole–cilium interface and facilitates proper cilium formation and function

YL Lee, J Santé, CJ Comerci, B Cyge… - Molecular Biology of …, 2014 - Am Soc Cell Biol
Defects in centrosome and cilium function are associated with phenotypically related
syndromes called ciliopathies. Cby1, the mammalian orthologue of the Drosophila Chibby …

STED imaging of tau filaments in Alzheimer's disease cortical grey matter

A Benda, H Aitken, DS Davies, R Whan… - Journal of structural …, 2016 - Elsevier
Alzheimer's disease (AD) involves the propagation of filaments of tau protein throughout the
cerebral cortex. Imaging tau filaments and oligomers in human brain at high resolution …

STED Imaging in Drosophila Brain Slices

S Fendl, J Pujol-Martí, J Ryan, A Borst… - Light Microscopy: Methods …, 2017 - Springer
Super-resolution microscopy is a very powerful tool to investigate fine cellular structures and
molecular arrangements in biological systems. For instance, stimulated emission depletion …

Nanostructure of the desmosomal plaque

I Iachina, H Gam-Hadberg, JR Brewer - bioRxiv, 2022 - biorxiv.org
Desmosomes are considered one of the most important intercellular junctions with respect to
mechanical strength. Therefore, their spatial distribution and structure is of interest with …

ONE-PHOTON FLUORESCENCE MICROSCOPY

D Wang, Y Tian, Y Gong - Biomedical Optical Imaging, 2021 - pubs.aip.org
The development of the one-photon fluorescence microscope traces from the turn of the first
millennium past the turn of the second millennium. The invention of the first optical …