Opportunities and challenges in design and optimization of protein function
The field of protein design has made remarkable progress over the past decade. Historically,
the low reliability of purely structure-based design methods limited their application, but …
the low reliability of purely structure-based design methods limited their application, but …
Principles and practical applications of structure-based vaccine design
PO Byrne, JS McLellan - Current opinion in immunology, 2022 - Elsevier
Highlights•Summary of the basic principles of structure-based vaccine design.•Review of
recent advances (the years 2019–2021) in structure-based vaccine design.•Preview of …
recent advances (the years 2019–2021) in structure-based vaccine design.•Preview of …
Mega-scale experimental analysis of protein folding stability in biology and design
Advances in DNA sequencing and machine learning are providing insights into protein
sequences and structures on an enormous scale. However, the energetics driving folding …
sequences and structures on an enormous scale. However, the energetics driving folding …
Advancing enzyme's stability and catalytic efficiency through synergy of force-field calculations, evolutionary analysis, and machine learning
Thermostability is an essential requirement for the use of enzymes in the bioindustry. Here,
we compare different protein stabilization strategies using a challenging target, a stable …
we compare different protein stabilization strategies using a challenging target, a stable …
Stable and functionally diverse versatile peroxidases designed directly from sequences
White-rot fungi secrete a repertoire of high-redox potential oxidoreductases to efficiently
decompose lignin. Of these enzymes, versatile peroxidases (VPs) are the most promiscuous …
decompose lignin. Of these enzymes, versatile peroxidases (VPs) are the most promiscuous …
Designed high-redox potential laccases exhibit high functional diversity
White-rot fungi secrete an impressive repertoire of high-redox potential laccases (HRPLs)
and peroxidases for efficient oxidation and utilization of lignin. Laccases are attractive …
and peroxidases for efficient oxidation and utilization of lignin. Laccases are attractive …
Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains
JY Wang, A Khmelinskaia, W Sheffler… - Proceedings of the …, 2023 - National Acad Sciences
Computationally designed protein nanoparticles have recently emerged as a promising
platform for the development of new vaccines and biologics. For many applications …
platform for the development of new vaccines and biologics. For many applications …
Tools for computational design and high-throughput screening of therapeutic enzymes
Therapeutic enzymes are valuable biopharmaceuticals in various biomedical applications.
They have been successfully applied for fibrinolysis, cancer treatment, enzyme replacement …
They have been successfully applied for fibrinolysis, cancer treatment, enzyme replacement …
Enhancing thermostability of lipase from Pseudomonas alcaligenes for producing l-menthol by the CREATE strategy
A lipase from Pseudomonas alcaligenes (PaL) catalyzes the hydrolysis of racemic menthol
propionate to produce L-menthol, one of the most important flavouring agents in the food …
propionate to produce L-menthol, one of the most important flavouring agents in the food …
[HTML][HTML] A concise guide to choosing suitable gene expression systems for recombinant protein production
A Schütz, F Bernhard, N Berrow, JF Buyel… - STAR protocols, 2023 - Elsevier
This overview guides both novices and experienced researchers facing challenging targets
to select the most appropriate gene expression system for producing a particular protein. By …
to select the most appropriate gene expression system for producing a particular protein. By …