[HTML][HTML] The centenary of the Stern-Volmer equation of fluorescence quenching: From the single line plot to the SV quenching map

MH Gehlen - Journal of Photochemistry and Photobiology C …, 2020 - Elsevier
In the year of 2019, we celebrate the centenary of the publication of the remarkable paper by
Otto Stern and Max Volmer concerning the kinetics analysis of fluorescence quenching …

Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications

J Sankaran, T Wohland - Communications Biology, 2023 - nature.com
Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the
quantitative measurement of biomolecular dynamics and interactions. Improvements in …

Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms

JW Krieger, AP Singh, N Bag, CS Garbe… - Nature protocols, 2015 - nature.com
Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes
can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved …

Single-Molecule Spectroscopy and Super-Resolution Mapping of Physicochemical Parameters in Living Cells

MA Steves, C He, K Xu - Annual Review of Physical Chemistry, 2024 - annualreviews.org
By superlocalizing the positions of millions of single molecules over many camera frames, a
class of super-resolution fluorescence microscopy methods known as single-molecule …

Carbon quantum dot-gold nanocluster nanosatellite for ratiometric fluorescence probe and imaging for hydrogen peroxide in living cells

Z Li, S Guo, Z Yuan, C Lu - Sensors and Actuators B: Chemical, 2017 - Elsevier
Developing probes for sensitive H 2 O 2 detection is critical for understanding its related
physiological and pathological processes. In this study, novel carbon quantum dot-gold …

Bacterial vivisection: how fluorescence-based imaging techniques shed a light on the inner workings of bacteria

A Cambré, A Aertsen - Microbiology and Molecular Biology …, 2020 - Am Soc Microbiol
The rise in fluorescence-based imaging techniques over the past 3 decades has improved
the ability of researchers to scrutinize live cell biology at increased spatial and temporal …

Plasma membrane organization of epidermal growth factor receptor in resting and ligand-bound states

N Bag, S Huang, T Wohland - Biophysical journal, 2015 - cell.com
The spatial arrangement of the epidermal growth factor receptor (EGFR) on the cellular
plasma membrane is one of the prime factors that control its downstream signaling pathways …

Ultrasmall, Bright, and Photostable Fluorescent Core–Shell Aluminosilicate Nanoparticles for Live‐Cell Optical Super‐Resolution Microscopy

JA Erstling, JA Hinckley, N Bag, J Hersh… - Advanced …, 2021 - Wiley Online Library
Stochastic optical reconstruction microscopy (STORM) is an optical super‐resolution
microscopy (SRM) technique that traditionally requires toxic and non‐physiological imaging …

Quantifying biomolecular organisation in membranes with brightness-transit statistics

F Schneider, PF Cespedes, N Karedla… - Nature …, 2024 - nature.com
Cells crucially rely on the interactions of biomolecules at their plasma membrane to maintain
homeostasis. Yet, a methodology to systematically quantify biomolecular organisation …

Multimodal light microscopy approaches to reveal structural and functional properties of promyelocytic leukemia nuclear bodies

C Hoischen, S Monajembashi, K Weisshart… - Frontiers in …, 2018 - frontiersin.org
The promyelocytic leukemia (pml) gene product PML is a tumor suppressor localized mainly
in the nucleus of mammalian cells. In the cell nucleus, PML seeds the formation of …