Environmental sensing of heavy metals through whole cell microbial biosensors: a synthetic biology approach

LT Bereza-Malcolm, G Mann, AE Franks - ACS synthetic biology, 2015 - ACS Publications
Whole cell microbial biosensors are offering an alternative means for rapid, on-site heavy
metal detection. Based in microorganisms, biosensing constructs are designed and …

Modular tuning engineering and versatile applications of genetically encoded biosensors

J Zhang, Q Pang, Q Wang, Q Qi… - Critical Reviews in …, 2022 - Taylor & Francis
Genetically encoded biosensors have a diverse range of detectable signals and potential
applications in many fields, including metabolism control and high-throughput screening …

In vivo biosensors: mechanisms, development, and applications

S Shi, EL Ang, H Zhao - Journal of Industrial Microbiology and …, 2018 - academic.oup.com
In vivo biosensors can recognize and respond to specific cellular stimuli. In recent years,
biosensors have been increasingly used in metabolic engineering and synthetic biology …

Whole‐cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production

L Goers, C Ainsworth, CH Goey… - Biotechnology and …, 2017 - Wiley Online Library
Many high‐value added recombinant proteins, such as therapeutic glycoproteins, are
produced using mammalian cell cultures. In order to optimize the productivity of these …

Sense and sensitivity in bioprocessing—detecting cellular metabolites with biosensors

L Dekker, KM Polizzi - Current opinion in chemical biology, 2017 - Elsevier
Highlights•Recent developments in metabolite biosensors important for bioprocessing are
reviewed.•Industry still mainly employs electrochemical biosensor analyzers.•Advances in …

Computational re-design of synthetic genetic oscillators for independent amplitude and frequency modulation

M Tomazou, M Barahona, KM Polizzi, GB Stan - Cell systems, 2018 - cell.com
To perform well in biotechnology applications, synthetic genetic oscillators must be
engineered to allow independent modulation of amplitude and period. This need is currently …

Characterization and designing of an SAM riboswitch to establish a high‐throughput screening platform for SAM overproduction in Saccharomyces cerevisiae

X Fu, X Zuo, X Zhao, H Zhang… - Biotechnology and …, 2023 - Wiley Online Library
Abstract S‐adenosyl‐l‐methionine (SAM) is a high‐value compound widely used in the
treatment of various diseases. SAM can be produced through fermentation, but further …

Direct evolution of riboflavin kinase significantly enhance flavin mononucleotide synthesis by design and optimization of flavin mononucleotide riboswitch

Y Du, X Zhang, H Zhang, R Zhu, Z Zhao, J Han… - Bioresource …, 2023 - Elsevier
Flavin mononucleotide (FMN) is the active form of riboflavin. It has a wide range of
application scenarios in the pharmaceutical and food additives. However, there are …

Genetic circuits to detect nanomaterial triggered toxicity through engineered heat shock response mechanism

B Saltepe, EU Bozkurt, N Hacıosmanoğlu… - ACS Synthetic …, 2019 - ACS Publications
Biocompatibility assessment of nanomaterials has been of great interest due to their
potential toxicity. However, conventional biocompatibility tests fall short of providing a fast …

Fundamental building blocks of whole-cell biosensor design

KY Wen, JW Rutter, CP Barnes, L Dekker - Handbook of cell biosensors, 2022 - Springer
Whole-cell biosensors are one of the most popular applications of synthetic biology.
Biological sensing mechanisms originating from natural systems are coupled to response …