Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer

L Dalla Costa, S Piazza, V Pompili, U Salvagnin… - Scientific Reports, 2020 - nature.com
Genome editing via CRISPR/Cas9 is a powerful technology, which has been widely applied
to improve traits in cereals, vegetables and even fruit trees. For the delivery of CRISPR/Cas9 …

Application of next-generation sequencing in the detection of transgenic crop

S He, Y Fan, S Tao, Y Zhang, C Yin, X Yu - Frontiers in Genetics, 2024 - frontiersin.org
With the rapid development of transgenic technology and the increasing prevalence of
genetically modified (GMO) crops, incidents such as illegal importation, environmental …

Next-generation sequencing technology: a boon to agriculture

B Marudamuthu, T Sharma, S Purru, SK Soam… - Genetic Resources and …, 2023 - Springer
Next-generation sequencing (NGS) is a massively parallel sequencing technology that has
revolutionized genomic research. The goal of genomics is outlining the genetic composition …

Comparison of qPCR and metabarcoding methods as tools for the detection of airborne inoculum of forest fungal pathogens

A Chandelier, J Hulin, G San Martin… - …, 2021 - Am Phytopath Society
Forest diseases caused by invasive fungal pathogens are becoming more common,
sometimes with dramatic consequences to forest ecosystems. The development of early …

Targeted high-throughput sequencing enables the detection of single nucleotide variations in CRISPR/Cas9 gene-edited organisms

MA Fraiture, J D'aes, E Guiderdoni, AC Meunier… - Foods, 2023 - mdpi.com
Similar to genetically modified organisms (GMOs) produced by classical genetic
engineering, gene-edited (GE) organisms and their derived food/feed products …

Roadmap for the integration of environmental microbiomes in risk assessments under EFSA's remit

F Debode, S Caulier, S Demeter… - EFSA Supporting …, 2024 - Wiley Online Library
Scientific interest in the use of environmental microbiomes for risk assessment is rapidly
growing, as exemplified by various EFSA opinions. In the absence of official regulatory …

An integrated strategy involving high‐throughput sequencing to characterize an unknown GM wheat event in Canada

MC Gagnon, MO Duceppe, A Colville… - Plant Biotechnology …, 2024 - Wiley Online Library
Glyphosate‐resistant wheat plants were discovered in southern Alberta in 2017,
representing an unauthorized GM release in Canada. The Canadian Food Inspection …

Transcriptomic Analysis of Mature Transgenic Poplar Expressing the Transcription Factor JERF36 Gene in Two Different Environments

W Zhang, Y Wang, T Zhang, J Zhang, L Shen… - … in bioengineering and …, 2022 - frontiersin.org
During the last several decades, a number of transgenic or genetically modified tree
varieties with enhanced characteristics and new traits have been produced. These trees …

GmoDetector: An accurate and efficient GMO identification approach and its applications

L Chen, J Zhou, T Li, Z Fang, L Li, G Huang… - Food Research …, 2021 - Elsevier
The rapid increase of genetically modified organisms (GMOs) entering the food and feed
markets, and the contamination of donor (micro) organisms of transgenic elements make it …

LIFE‐Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified …

H Zhang, R Li, Y Guo, Y Zhang… - Plant Biotechnology …, 2022 - Wiley Online Library
Molecular characterization of genetically modified organisms (GMOs) yields basic
information on exogenous DNA integration, including integration sites, entire inserted …