[HTML][HTML] Metagenomic sequencing for microbial DNA in human samples: emerging technological advances

Y Shi, G Wang, HCH Lau, J Yu - International journal of molecular …, 2022 - mdpi.com
Whole genome metagenomic sequencing is a powerful platform enabling the simultaneous
identification of all genes from entirely different kingdoms of organisms in a complex sample …

Navigating clinical utilization of direct-from-specimen metagenomic pathogen detection: clinical applications, limitations, and testing recommendations

LM Filkins, AL Bryson, SA Miller, SL Mitchell - Clinical chemistry, 2020 - academic.oup.com
Background Metagenomic next generation sequencing (mNGS) is becoming increasingly
available for pathogen detection directly from clinical specimens. These tests use target …

[HTML][HTML] Shotgun sequencing of honey DNA can describe honey bee derived environmental signatures and the honey bee hologenome complexity

S Bovo, VJ Utzeri, A Ribani, R Cabbri, L Fontanesi - Scientific Reports, 2020 - nature.com
Honey bees are large-scale monitoring tools due to their extensive environmental
exploration. In their activities and from the hive ecosystem complex, they get in close contact …

Where have all the diagnostic morphological parasitologists gone?

RS Bradbury, SGH Sapp, I Potters… - Journal of clinical …, 2022 - Am Soc Microbiol
Advances in laboratory techniques have revolutionized parasitology diagnostics over the
past several decades. Widespread implementation of rapid antigen detection tests has …

Targeted metagenomic sequencing-based approach applied to 2146 tissue and body fluid samples in routine clinical practice

L Flurin, MJ Wolf, MM Mutchler… - Clinical Infectious …, 2022 - academic.oup.com
Background The yield of next-generation sequencing (NGS) added to a Sanger sequencing–
based 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) assay was …

[HTML][HTML] Evaluation of extraction and enrichment methods for recovery of respiratory RNA viruses in a metagenomics approach

AE Ogunbayo, S Sabiu, MM Nyaga - Journal of Virological Methods, 2023 - Elsevier
Viral metagenomics is increasingly applied in viral detection and virome characterization.
Different extraction and enrichment techniques may be adopted, however, reports on their …

[HTML][HTML] Metagenomic shotgun sequencing of blood to identify bacteria and viruses in leukemic febrile neutropenia

P Vijayvargiya, A Feri, M Mairey, C Rouillon… - PloS one, 2022 - journals.plos.org
Despite diagnostic advances in microbiology, the etiology of neutropenic fever remains
elusive in most cases. In this study, we evaluated the utility of a metagenomic shotgun …

Insights into microbial diversity on plastisphere by multi-omics

N Tiwari, M Bansal, D Santhiya, JG Sharma - Archives of Microbiology, 2022 - Springer
Plastic pollution is a major concern in marine environment as it takes many years to degrade
and is one of the greatest threats to marine life. Plastic surface, referred to as plastisphere …

The microbiota in hematologic malignancies

Y Song, B Himmel, L Öhrmalm, P Gyarmati - Current Treatment Options in …, 2020 - Springer
Opinion statement There are approximately 1.2 million new hematologic malignancy cases
resulting in~ 690,000 deaths each year worldwide, and hematologic malignancies remain …

[HTML][HTML] Whole-genome sequencing analysis to identify infection with multiple species of nontuberculous mycobacteria

V Khieu, P Ananta, O Kaewprasert, M Laohaviroj… - Pathogens, 2021 - mdpi.com
Mixed infection with multiple species of nontuberculous mycobacteria (NTM) is difficult to
identify and to treat. Current conventional molecular-based methods for identifying mixed …