DNA transformation

C Mello, A Fire - Methods in cell biology, 1995 - Elsevier
Publisher Summary This chapter discusses DNA transformation. DNA transformation assays
in a whole organism provide experimental links between molecular structure and …

Protein-protein interactions: methods for detection and analysis

EM Phizicky, S Fields - Microbiological reviews, 1995 - Am Soc Microbiol
The function and activity of a protein are often modulated by other proteins with which it
interacts. This review is intended as a practical guide to the analysis of such protein-protein …

On the role of RNA amplification in dsRNA-triggered gene silencing

T Sijen, J Fleenor, F Simmer, KL Thijssen, S Parrish… - Cell, 2001 - cell.com
We have investigated the role of trigger RNA amplification during RNA interference (RNAi)
in Caenorhabditis elegans. Analysis of small interfering RNAs (siRNAs) produced during …

Caenorhabditis elegans levamisole resistance geneslev-1, unc-29, and unc-38 encode functional nicotinic acetylcholine receptor subunits

JT Fleming, MD Squire, TM Barnes… - Journal of …, 1997 - Soc Neuroscience
We show that three of the eleven genes of the nematode Caenorhabditis elegans that
mediate resistance to the nematocide levamisole and to other cholinergic agonists encode …

Functional anatomy of a dsRNA trigger: differential requirement for the two trigger strands in RNA interference

S Parrish, J Fleenor, SQ Xu, C Mello, A Fire - Molecular cell, 2000 - cell.com
In RNA-mediated interference (RNAi), externally provided mixtures of sense and antisense
RNA trigger concerted degradation of homologous cellular RNAs. We show that RNAi …

Mechanoenzymatics of titin kinase

EM Puchner, A Alexandrovich, AL Kho… - Proceedings of the …, 2008 - National Acad Sciences
Biological responses to mechanical stress require strain-sensing molecules, whose
mechanically induced conformational changes are relayed to signaling cascades mediating …

Titin: properties and family relationships

L Tskhovrebova, J Trinick - Nature reviews Molecular cell biology, 2003 - nature.com
In striated muscles, the rapid production of macroscopic levels of force and displacement
stems directly from highly ordered and hierarchical protein organization, with the sarcomere …

[HTML][HTML] Development, structure, and maintenance of C. elegans body wall muscle

K Gieseler, H Qadota, GM Benian - … the online review of C. elegans …, 2018 - ncbi.nlm.nih.gov
In C. elegans, mutants that are defective in muscle function and/or structure are easy to
detect and analyze since: 1) body wall muscle is essential for locomotion, and 2) muscle …

Targeted mutagenesis in a human-parasitic nematode

SS Gang, ML Castelletto, AS Bryant, E Yang… - PLoS …, 2017 - journals.plos.org
Parasitic nematodes infect over 1 billion people worldwide and cause some of the most
common neglected tropical diseases. Despite their prevalence, our understanding of the …

Immunofluorescence microscopy

DM Miller, DC Shakes - Methods in cell biology, 1995 - Elsevier
Publisher Summary This chapter provides a practical guide to immunofluorescence
microscopy of Caenorhabditis elegans. In this method, fixed tissue is stained with a …