Recombinant protein production and purification of insoluble proteins

N Ferrer-Miralles, P Saccardo, JL Corchero… - … Proteins: Methods and …, 2022 - Springer
Proteins are synthesized in heterologous systems because of the impossibility to obtain
satisfactory yields from natural sources. The efficient production of soluble and functional …

Lactiplantibacillus plantarum: a new example of inclusion body producing bacteria

R Baltà-Foix, C Serrano-Adrover, A López-Cano… - Microbial Cell …, 2023 - Springer
Abstract Background Lactic Acid Bacteria such as Lactococcus lactis, Latilactobacillus sakei
(basonym: Lactobacillus sakei) and Lactiplantibacillus plantarum (basonym: Lactobacillus …

Potential of MMP-9 based nanoparticles at optimizing the cow dry period: Pulling apart the effects of MMP-9 and nanoparticles

L Gifre-Renom, JV Carratalá, S Parés… - Scientific reports, 2020 - nature.com
The cow dry period is a non-milking interval where the mammary gland involutes and
regenerates to guarantee an optimal milk production in the subsequent lactation. Important …

Selecting subpopulations of high-quality protein conformers among conformational mixtures of recombinant bovine mmp-9 solubilized from inclusion bodies

JV Carratalá, L Gifre-Renom, R Roca-Pinilla… - International journal of …, 2021 - mdpi.com
A detailed workflow to analyze the physicochemical characteristics of mammalian matrix
metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion …

Nondenaturing solubilization of inclusion bodies from lactic acid bacteria

L Gifre-Renom, R Baltà-Foix, A Arís… - … Proteins: Methods and …, 2022 - Springer
Since inclusion bodies (IBs) contain an important amount of properly folded and active
proteins, their solubilization using nondenaturing conditions to obtain aggregation-prone …

Functional inclusion bodies

R Baltà-Foix, R Roca-Pinilla, A López-Cano… - Microbial Production of …, 2022 - Springer
Inclusion bodies (IBs) are protein aggregates formed under recombinant protein production
processes in microbial cell factories. Their characterization has shown that they are self …

The future of recombinant host defense peptides

R Roca-Pinilla, L Lisowski, A Arís… - Microbial Cell …, 2022 - Springer
The antimicrobial resistance crisis calls for the discovery and production of new
antimicrobials. Host defense peptides (HDPs) are small proteins with potent antibacterial …

Antimicrobial Applications of Inclusion Bodies

R Roca-Pinilla, A Arís, E Garcia-Fruitós - Inclusion Bodies: Methods and …, 2023 - Springer
A broad number of inclusion bodies (IBs) potential uses, including biocatalysis,
biocompatible nanomaterials, and nanopills for biomedicine, have been described so far …

Development and characterization of protein nanoformulations as alternative therapeutics to reduce antibiotic usage

JV Carratalá - 2021 - ddd.uab.cat
The emergence of bacterial drug resistance to conventional antibiotics is a global alarming
situation. This worrying scenario has forced the implementation of measures such us …