High-throughput RNA sequencing (RNA-seq) is considered a powerful tool for novel gene
discovery and fine-tuned transcriptional profiling. The digital nature of RNA-seq is also …

Viruses have developed sophisticated biochemical and genetic mechanisms to manipulate
and exploit their hosts. Enzymes derived from viruses have been essential research tools …

T4 RNA ligases are commonly used to attach adapters to RNAs, but large differences in
ligation efficiency make detection and quantitation problematic. We developed a ligation …

Background The use of nucleic acid-modifying enzymes has driven the rapid advancement
in molecular biology. Understanding their function is important for modifying or improving …

Transposable elements (TEs) are mobile sequences of DNA that can become
transcriptionally active as an animal ages. Whether TE activity is simply a by-product of …

Here we describe a protocol for NAD captureSeq that allows for the identification of
nicotinamide-adenine dinucleotide (NAD)-capped RNA sequences in total RNA samples …

RNA ligases are important enzymes in molecular biology and are highly useful for the
manipulation and analysis of nucleic acids, including adapter ligation in next-generation …

Eukaryotic regulatory small RNAs (sRNAs) play significant roles in many fundamental
cellular processes. As such, they have emerged as useful biomarkers for diseases and cell …

The preparation of small RNA cDNA sequencing libraries depends on the unbiased ligation
of adapters to the RNA ends. Small RNA with 5'recessed ends are poor substrates for …

Nucleic acid fragment analysis via separation and detection are routine operations in
molecular biology. However, analysis of small single-stranded nucleic acid fragments (< …