In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

L-5-hydroxytryptophan (5-HTP) is both a drug and a natural component of some dietary
supplements. 5-HTP is produced from tryptophan by tryptophan hydroxylase (TPH), which is …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Biopharmaceuticals are one of the fastest-growing sectors in the biotechnology industry.
Within the umbrella of biopharmaceuticals, the biosimilar segment is expanding with …

Objective Glycation is a non-enzymatic and spontaneous post-translational modification
(PTM) generated by the reaction between reducing sugars and primary amine groups within …

The clinical efficacy and safety of protein-based drugs such as monoclonal antibodies
(mAbs) rely on the integrity of the protein higher order structure (HOS) during product …

UV and ambient light-induced modifications and related degradation of therapeutic proteins
are observed during manufacturing and storage. Therefore, to ensure product quality …

Adeno-associated viruses (AAVs) are commonly used as vectors for the delivery of gene
therapy targets. Characterization of AAV capsid proteins (VPs) and their post-translational …

The higher order structure (HOS) of monoclonal antibodies (mAbs) is an important quality
attribute with strong contribution to clinically relevant biological functions and drug safety …

The α-Gal epitope consisting of the terminal trisaccharide Galα1, 3Galβ1, 4GlcNAc exposed
on cell or protein surfaces can cause severe immune reactions, such as hypersensitivity …