Fluorescence resonance energy transfer (FRET)-based biosensors are effective analytical
tools extensively used in fields of biomedicine, pharmacology, toxicology, and food …

Enzyme‐catalysed site‐specific protein modifications enable the precision manufacture of
conjugates for the study of protein function and/or for therapeutic or diagnostic applications …

Enzymatic peptide ligation holds great promise in the study of protein functions and
development of protein therapeutics. Owing to their high catalytic efficiency and a minimal …

Peptide asparaginyl ligases (PALs) catalyze transpeptidation at the Asn residue of a short
Asn-Xaa1-Xaa2 tripeptide motif. Due to their high catalytic activity toward the P1-Asn …

Although numerous genetic, chemical, and physical strategies have been developed to
remodel the cell surface landscape for basic research and the development of live cell …

Background: Protein theranostics integrate both diagnostic and treatment functions on a
single disease-targeting protein. However, the preparation of these multimodal agents …

Transpeptidases are powerful tools for protein engineering but are largely restricted to
acting at protein backbone termini. Alternative enzymatic approaches for internal protein …

Peptide asparaginyl ligases (PALs) are useful tools for precision modifications of proteins
and live‐cell surfaces by ligating peptides after Asn/Asp (Asx). They share high sequence …

Asparaginyl endopeptidases (AEPs) are cysteinyl enzymes naturally catalyzing the
hydrolysis and transpeptidation reactions at Asx–Xaa bonds. These reactions go by a …

Plant legumains are Asn/Asp-specific endopeptidases that have diverse functions in plants.
Peptide asparaginyl ligases (PALs) are a special legumain subtype that primarily catalyze …