Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …

Horizontal gene transfer can trigger rapid shifts in bacterial evolution. Driven by a variety of
mobile genetic elements—in particular bacteriophages and plasmids—the ability to share …

Transcription-coupled DNA repair (TCR) is presumed to be a minor sub-pathway of
nucleotide excision repair (NER) in bacteria. Global genomic repair is thought to perform the …

Background As engineered biological systems become more complex, it is increasingly
common to express multiple operons from different plasmids and inducible expression …

Accurate measurements of promoter activities are crucial for predictably building genetic
systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts …

The internal environment of growing cells is variable and dynamic, making it difficult to
introduce reliable parts, such as promoters, for genetic engineering. Here, we applied …

Protein fermentation by the gut microbiota releases in the large intestine lumen various
amino-acid derived metabolites. Among them, hydrogen sulfide (H 2 S) in excess has been …

Models of transcription are often built around a picture of RNA polymerase and transcription
factors (TFs) acting on a single copy of a promoter. However, most TFs are shared between …

Plasmids are thought to play a key role in bacterial evolution by acting as vehicles for
horizontal gene transfer, but the role of plasmids as catalysts of gene evolution remains …