Identification and confirmation of bioactive small‐molecule targets is a crucial, often decisive
step both in academic and pharmaceutical research. Through the development and …

Congenital disorders of glycosylation (CDG) are a rapidly expanding group of metabolic
disorders that result from abnormal protein or lipid glycosylation. They are often difficult to …

Gene expression profiling by high-throughput sequencing reveals qualitative and
quantitative changes in RNA species at steady state but obscures the intracellular dynamics …

Identification of genes influencing a phenotype of interest is frequently achieved through
genetic screening by RNA interference (RNAi) or knockouts. However, RNAi may only …

Near-haploid human cell lines are instrumental for genetic screens and genome
engineering as gene inactivation is greatly facilitated by the absence of a second gene copy …

In mammals, the noncoding Xist RNA triggers transcriptional silencing of one of the two X
chromosomes in female cells. Here, we report a genetic screen for silencing factors in X …

Our understanding of the genetic mechanisms that underlie biological processes has relied
extensively on loss-of-function (LOF) analyses. LOF methods target DNA, RNA or protein to …

Mutations in the ATM tumor suppressor gene confer hypersensitivity to DNA-damaging
chemotherapeutic agents. To explore genetic resistance mechanisms, we performed …

Viruses depend on their hosts to complete their replication cycles; they exploit cellular
receptors for entry and hijack cellular functions to replicate their genome, assemble progeny …

Adeno-associated virus (AAV) is a highly promising gene transfer vector, yet major cellular
requirements for AAV entry are poorly understood. Using a genome-wide CRISPR screen …