Edible particle-stabilized water-in-water emulsions: Stabilization mechanisms, particle types, interfacial design, and practical applications
S Yan, JM Regenstein, S Zhang, Y Huang, B Qi, Y Li - Food Hydrocolloids, 2023 - Elsevier
Water-in-water emulsions are formed due to the thermodynamic incompatibility between
their components, which leads to phase separation. Water-in-water emulsions have many …
their components, which leads to phase separation. Water-in-water emulsions have many …
[HTML][HTML] It is time to crowd your cell culture media–physicochemical considerations with biological consequences
D Tsiapalis, DI Zeugolis - Biomaterials, 2021 - Elsevier
In vivo, the interior and exterior of cells is populated by various macromolecules that create
an extremely crowded milieu. Yet again, in vitro eukaryotic cell culture is conducted in dilute …
an extremely crowded milieu. Yet again, in vitro eukaryotic cell culture is conducted in dilute …
Understanding the intricacy of protein in hydrated deep eutectic solvent: Solvation dynamics, conformational fluctuation dynamics, and stability
Deep eutectic solvents (DESs) are potential biocatalytic media due to their easy preparation,
fine-tuneability, biocompatibility, and most importantly, due to their ability to keep protein …
fine-tuneability, biocompatibility, and most importantly, due to their ability to keep protein …
From enzyme stability to enzymatic bioelectrode stabilization processes
C Beaufils, HM Man, A de Poulpiquet, I Mazurenko… - Catalysts, 2021 - mdpi.com
Bioelectrocatalysis using redox enzymes appears as a sustainable way for biosensing,
electricity production, or biosynthesis of fine products. Despite advances in the knowledge of …
electricity production, or biosynthesis of fine products. Despite advances in the knowledge of …
Role of Associated Water Dynamics on Protein Stability and Activity in Crowded Milieu
Macromolecular crowding bridges in vivo and in vitro studies by simulating cellular
complexities such as high viscosity and limited space while maintaining the experimental …
complexities such as high viscosity and limited space while maintaining the experimental …
Transforming eukaryotic cell culture with macromolecular crowding
M Raghunath, DI Zeugolis - Trends in biochemical sciences, 2021 - cell.com
In multicellular organisms, the intracellular and extracellular spaces are considerably
packed with a diverse range of macromolecular species. Yet, standard eukaryotic cell …
packed with a diverse range of macromolecular species. Yet, standard eukaryotic cell …
Macromolecular crowding: how shape and interaction affect the structure, function, conformational dynamics and relative domain movement of a multi-domain protein
The cellular environment is crowded by macromolecules of various sizes, shapes, and
charges, which modulate protein structure, function and dynamics. Herein, we contemplated …
charges, which modulate protein structure, function and dynamics. Herein, we contemplated …
Correlating Bromelain's activity with its structure and active-site dynamics and the medium's physical properties in a hydrated deep eutectic solvent
Deep eutectic solvents (DESs) are emerging as new media of choice for biocatalysis due to
their environmentally friendly nature, fine-tunability, and potential biocompatibility. This work …
their environmentally friendly nature, fine-tunability, and potential biocompatibility. This work …
Shape-dependent macromolecular crowding on the thermodynamics and microsecond conformational dynamics of protein unfolding revealed at the single-molecule …
One of the main differences in the intercellular environment compared to the laboratory
condition is the presence of macromolecular crowders of various compositions, sizes, and …
condition is the presence of macromolecular crowders of various compositions, sizes, and …
Associated water dynamics might be a key factor affecting protein stability in the crowded milieu
Over the past 20 years, the most studied and debated aspect of macromolecular crowding is
how it affects protein stability. Traditionally, it is explained by a delicate balance between the …
how it affects protein stability. Traditionally, it is explained by a delicate balance between the …