[HTML][HTML] Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications–a systematic review

M Leifels, D Cheng, E Sozzi, DC Shoults, S Wuertz… - Water Research X, 2021 - Elsevier
Capsid integrity quantitative PCR (qPCR), a molecular detection method for infectious
viruses combining azo dye pretreatment with qPCR, has been widely used in recent years; …

Several forms of SARS-CoV-2 RNA can be detected in wastewaters: implication for wastewater-based epidemiology and risk assessment

S Wurtzer, P Waldman, A Ferrier-Rembert… - Water Research, 2021 - Elsevier
The ongoing global pandemic of coronavirus disease 2019 (COVID-19) caused by severe
acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a public health …

A systematic review and meta-analysis indicates a substantial burden of human noroviruses in shellfish worldwide, with GII. 4 and GII. 2 being the predominant …

Y Li, L Xue, J Gao, W Cai, Z Zhang, L Meng, S Miao… - Food …, 2023 - Elsevier
Human noroviruses (HuNoVs) have been found as the leading cause of acute
gastroenteritis outbreaks in all age groups and are significantly correlated with the …

Challenges for estimating human norovirus infectivity by viability RT-qPCR as compared to replication in human intestinal enteroids

SQ Wales, A Pandiscia, M Kulka, G Sanchez… - International journal of …, 2024 - Elsevier
Viability RT-qPCR, a molecular detection method combining viability marker pre-treatment
with RT-qPCR, has been proposed to infer infectivity of viruses which is particularly relevant …

[HTML][HTML] Detection of SARS-CoV-2 RNA in bivalve mollusks and marine sediments

D Polo, M Lois, MT Fernández-Núñez… - Science of the Total …, 2021 - Elsevier
The presence of SARS-CoV-2 in wastewater pose the question of whether this new
pandemic virus could be released into watercourses and potentially continue to finally reach …

Epidemiological surveillance of norovirus and rotavirus in sewage (2016–2017) in Valencia (Spain)

C Santiso-Bellón, W Randazzo, A Pérez-Cataluña… - Microorganisms, 2020 - mdpi.com
The aim of the present study was to perform the molecular epidemiology of rotaviruses and
noroviruses detected in sewage samples from a large wastewater facility from the city of …

Detection of viable but nonculturable Vibrio parahaemolyticus in shrimp samples using improved real-time PCR and real-time LAMP methods

X Cao, L Zhao, J Zhang, X Chen, L Shi, X Fang, H Xie… - Food Control, 2019 - Elsevier
Accurate and highly sensitive detection of viable but nonculturable (VBNC) Vibrio
parahaemolyticus is crucial for preventing seafood-related outbreaks. The VBNC state may …

Rapid selective detection of potentially infectious porcine epidemic diarrhea coronavirus exposed to heat treatments using viability RT-qPCR

H Puente, W Randazzo, I Falcó, A Carvajal… - Frontiers in …, 2020 - frontiersin.org
Coronaviruses (CoVs) cause severe respiratory, enteric, and systemic infections in a wide
range of hosts, including humans and animals. Porcine epidemic diarrhea virus (PEDV), a …

Monitoring human viral pathogens reveals potential hazard for treated wastewater discharge or reuse

E Cuevas-Ferrando, A Pérez-Cataluña, I Falcó… - Frontiers in …, 2022 - frontiersin.org
Wastewater discharge to the environment or its reuse after sanitization poses a concern for
public health given the risk of transmission of human viral diseases. However, estimating the …

Optimisation of a PMAxx™-RT-qPCR assay and the preceding extraction method to selectively detect infectious murine norovirus particles in mussels

RM Razafimahefa, LF Ludwig-Begall… - Food and Environmental …, 2021 - Springer
Human noroviruses are a major cause for gastroenteritis outbreaks. Filter-feeding bivalve
molluscs, which accumulate noroviruses in their digestive tissues, are a typical vector for …