Intracellular delivery by membrane disruption: mechanisms, strategies, and concepts

MP Stewart, R Langer, KF Jensen - Chemical reviews, 2018 - ACS Publications
Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

A comprehensive review on intracellular delivery

D Morshedi Rad, M Alsadat Rad… - Advanced …, 2021 - Wiley Online Library
Intracellular delivery is considered an indispensable process for various studies, ranging
from medical applications (cell‐based therapy) to fundamental (genome‐editing) and …

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

P Zhang, N Shao, L Qin - Advanced Materials, 2021 - Wiley Online Library
Cell‐based living materials, including single cells, cell‐laden fibers, cell sheets, organoids,
and organs, have attracted intensive interests owing to their widespread applications in …

Non-viral transfection technologies for next-generation therapeutic T cell engineering

L Raes, SC De Smedt, K Raemdonck… - Biotechnology …, 2021 - Elsevier
Genetically engineered T cells have sparked interest in advanced cancer treatment,
reaching a milestone in 2017 with two FDA-approvals for CD19-directed chimeric antigen …

Delivering the CRISPR/Cas9 system for engineering gene therapies: Recent cargo and delivery approaches for clinical translation

RA Foley, RA Sims, EC Duggan, JK Olmedo… - … in Bioengineering and …, 2022 - frontiersin.org
Clustered Regularly Interspaced Short Palindromic Repeats associated protein 9
(CRISPR/Cas9) has transformed our ability to edit the human genome selectively. This …

Intracellular delivery of nanomaterials via an inertial microfluidic cell hydroporator

Y Deng, M Kizer, M Rada, J Sage, X Wang… - Nano …, 2018 - ACS Publications
The introduction of nanomaterials into cells is an indispensable process for studies ranging
from basic biology to clinical applications. To deliver foreign nanomaterials into living cells …

Microfluidic generation of transient cell volume exchange for convectively driven intracellular delivery of large macromolecules

A Liu, M Islam, N Stone, V Varadarajan, J Jeong… - Materials Today, 2018 - Elsevier
Efficient intracellular delivery of target macromolecules remains a major obstacle in cell
engineering and other biomedical applications. We discovered a unique cell biophysical …

Single-cell transfection technologies for cell therapies and gene editing

B Duckert, S Vinkx, D Braeken, M Fauvart - Journal of Controlled Release, 2021 - Elsevier
Advances in gene editing and cell therapies have recently led to outstanding clinical
successes. However, the lack of a cost-effective manufacturing process prevents the …

Intracellular labeling with extrinsic probes: delivery strategies and applications

J Liu, JC Fraire, SC De Smedt, R Xiong, K Braeckmans - Small, 2020 - Wiley Online Library
Extrinsic probes have outstanding properties for intracellular labeling to visualize dynamic
processes in and of living cells, both in vitro and in vivo. Since extrinsic probes are in many …

High‐Throughput and Dosage‐Controlled Intracellular Delivery of Large Cargos by an Acoustic‐Electric Micro‐Vortices Platform

M Aghaamoo, YH Chen, X Li, N Garg… - Advanced …, 2022 - Wiley Online Library
A high‐throughput non‐viral intracellular delivery platform is introduced for the transfection
of large cargos with dosage‐control. This platform, termed Acoustic‐Electric Shear Orbiting …