Several proteomics approaches are available that are defined by the level (protein or
peptide) at which analysis takes place. The most widely applied method still is bottom-up …

The role of mass spectrometry (MS) has become more important in most application
domains in recent years. Pharmaceutical analysis is specific due to its stringent regulation …

Characterization of unknown monoclonal antibody (mAb) variants is important in order to
identify their potential impact on safety, potency, and stability. Ion exchange chromatography …

In this work, a novel and facile route was developed for the immobilization of enzyme on
nanosized magnetic particles, and its application to fast protein digestion via a direct MALDI …

Fast, efficient characterization of proteins is becoming one of the hottest topics in the
bioanalytical community, especially for large-scale proteomic studies. As an attractive …

Poly (glycidylmethacrylate-methylmethacrylate), poly (GMA-MMA) beads were prepared via
suspension polymerization in the presence of ferric ions. The epoxy groups of the poly (GMA …

A fast and efficient proteolysis approach of microwave-assisted protein digestion was
developed by using trypsin-immobilized magnetic silica (MS) microspheres. In the work …

Immobilized enzymatic reactors recently have drawn much attention because of the striking
advantages, such as high substrate turnover rate and ease in coupling with the separation …

We describe the preparation of a capillary trypsin immobilized monolithic enzyme reactor
(IMER) for a rapid and efficient digestion of proteins down to the femtomole level. Trypsin …

In the current proteome study, protein digestion is indispensable before proteins could be
identified by MS/MS, no matter based on top–down or bottom–up strategies. Compared to …