Development of diagnostic tests provides technical support for the control of African swine fever

Z Qiu, Z Li, Q Yan, Y Li, W Xiong, K Wu, X Li, S Fan… - Vaccines, 2021 - mdpi.com
African swine fever is a highly contagious global disease caused by the African swine fever
virus. Since African swine fever (ASF) was introduced to Georgia in 2007, it has spread to …

Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics

Y Zhu, M Zhang, Z Jie, S Guo, Z Zhu, S Tao - Veterinary Research, 2024 - Springer
African swine fever (ASF) is a devastating disease caused by African swine fever virus
(ASFV) and leads to significant economic losses in the pig farming industry. Given the …

Fluorescence and colorimetric analysis of African swine fever virus based on the RPA-assisted CRISPR/Cas12a strategy

G Mao, X Luo, S Ye, X Wang, J He, J Kong… - Analytical …, 2023 - ACS Publications
It is well-established that different detection modes are necessary for corresponding
applications, which can effectively reduce matrix interference and improve the detection …

LAMP assay coupled with CRISPR/Cas12a system for portable detection of African swine fever virus

B Yang, Z Shi, Y Ma, L Wang, L Cao… - Transboundary and …, 2022 - Wiley Online Library
African swine fever (ASF) is one of the most severe infectious diseases of pigs. In this study,
a loop‐mediated isothermal amplification (LAMP) assay coupled with the clustered regularly …

Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues

S Cavalera, B Colitti, GM De Mia, F Feliziani… - Talanta, 2023 - Elsevier
African swine fever (ASF) is a severe haemorrhagic infectious disease affecting suids, thus
representing a great economic concern. Considering the importance of the early diagnosis …

Advanced strategies for developing vaccines and diagnostic tools for African swine fever

JW Lim, TTH Vu, VP Le, M Yeom, D Song, DG Jeong… - Viruses, 2023 - mdpi.com
African swine fever (ASF) is one of the most lethal infectious diseases affecting domestic
pigs and wild boars of all ages. Over a span of 100 years, ASF has continued to spread over …

Rapid detection of Toxoplasma gondii DNA in cat feces using colorimetric loop-mediated isothermal amplification (LAMP) assays targeting RE and B1 genes

M Karakavuk, H Can, T Karakavuk, A Gül… - … and Infectious Diseases, 2022 - Elsevier
Toxoplasma gondii is an obligate protozoan parasite that can infect mammals and birds.
Cats are the definitive host of T. gondii and have a very important role in transmission of …

Evaluation of a lateral flow assay for rapid detection of African swine fever virus in multiple sample types

C Onyilagha, K Nguyen, PD Luka, U Hussaini… - Pathogens, 2022 - mdpi.com
Antibody-based lateral flow assay (LFA) is a quick and inexpensive tool used to detect
pathogens in field samples, especially in hard-to-reach remote areas that may have limited …

[HTML][HTML] Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples

R Khangembam, M Tóth, N Vass, M Várady, L Czeglédi… - Parasite, 2021 - ncbi.nlm.nih.gov
In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the
detection of Haemonchus contortus in small ruminant faecal samples. Using a previously …

Development of a highly sensitive point‐of‐care test for African swine fever that combines EZ‐Fast DNA extraction with LAMP detection: Evaluation using naturally …

MT Ngan, H Thi My Le, V Xuan Dang… - Veterinary Medicine …, 2023 - Wiley Online Library
Background While early detection and early containment are key to controlling the African
swine fever (ASF) pandemic, the lack of practical testing methods for use in the field are a …