Unveiling intracellular phase separation: advances in optical imaging of biomolecular condensates
Y Guo, X Zhang - Trends in Biochemical Sciences, 2024 - cell.com
Intracellular biomolecular condensates, which form via phase separation, display a highly
organized ultrastructure and complex properties. Recent advances in optical imaging …
organized ultrastructure and complex properties. Recent advances in optical imaging …
Fundamental limits in measuring the anisotropic rotational diffusion of single molecules
Many biophysical techniques, such as single-molecule fluorescence correlation
spectroscopy, Förster resonance energy transfer, and fluorescence anisotropy, measure …
spectroscopy, Förster resonance energy transfer, and fluorescence anisotropy, measure …
Single-molecule orientation-localization microscopy: Applications and approaches
Single-molecule orientation-localization microscopy (SMOLM) builds upon super-resolved
localization microscopy by imaging orientations and rotational dynamics of individual …
localization microscopy by imaging orientations and rotational dynamics of individual …
[PDF][PDF] Single molecule tracking reveals nanodomains in biomolecular condensates
Protein-rich biomolecular condensates formed by phase separation1 play a crucial role in
cellular RNA regulation by the selective recruitment and processing of RNA molecules2. The …
cellular RNA regulation by the selective recruitment and processing of RNA molecules2. The …
6D single-fluorogen orientation-localization microscopy for elucidating the architecture of beta-sheet assemblies and biomolecular condensates
We develop six-dimensional single-molecule orientation-localization microscopy (SMOLM)
to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We …
to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We …