Harvesting, processing, and evaluation of in vitro-manipulated equine preantral follicles: A review

EL Gastal, FLN Aguiar, GDA Gastal, KA Alves… - Theriogenology, 2020 - Elsevier
The mammalian ovary is responsible for essential stages of folliculogenesis and hormonal
production, regulating the female physiological functions during the menstrual/estrous …

Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation

S Gebremedhn, A Gad, GM Ishak… - Molecular human …, 2023 - academic.oup.com
Innumerable similarities in reproductive cyclicity and hormonal alterations highlight the
considerable utility of the mare to study aspects of follicular dynamics and reproductive …

Follicular‐fluid proteomics during equine follicle development

GM Ishak, JM Feugang, O Pechanova… - Molecular …, 2022 - Wiley Online Library
The complex composition of the follicular fluid (FF), the intimate proximity to the oocyte, and
the continual changes in their composition have a major effect on folliculogenesis and …

Seasonal variation in equine follicular fluid proteome

GA Dutra, GM Ishak, O Pechanova, T Pechan… - Reproductive Biology …, 2019 - Springer
Background Proteomic studies of follicular fluid (FF) exist for several species, including the
horse; however, the seasonal influence on FF proteome has not been explored in livestock …

Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue

MA Borges, BR Curcio, GDA Gastal, L Gheno… - Reproductive …, 2023 - Elsevier
The most adequate fixative solution for equine ovarian tissue is still to be determined as a
tool to evaluate the improvement of methodological studies in assisted reproductive …

Hemodynamic, endocrine, and gene expression mechanisms regulating equine ovarian follicular and cellular development

A Wischral, M Pastorello, MO Gastal… - Molecular …, 2022 - Wiley Online Library
Ovulatory follicle development and associated oocyte maturation involve complex
coordinated molecular and cellular mechanisms not yet fully understood. This study …

Deficiency in proliferative, angiogenic, and LH receptors in the follicle wall: implications of season toward the anovulatory condition

GM Ishak, GA Dutra, GDA Gastal, ME Elcombe… - Domestic Animal …, 2020 - Elsevier
This study aimed to gain insight on the effect of different seasons of the year on the
expression pattern of growth factor and hormone receptors involved in follicle development …

Transition to the ovulatory season in mares: An investigation of antral follicle receptor gene expression in vivo

GMA Ishak, GA Dutra, GDA Gastal… - Molecular …, 2019 - Wiley Online Library
The inability to obtain in vivo samples of antral follicle wall layers without removing the
ovaries or sacrificing the animals has limited more in‐depth studies on folliculogenesis. In …

Effect of cryopreservation techniques on proliferation and apoptosis of cultured equine ovarian tissue

GDA Gastal, FLN Aguiar, GM Ishak, CA Cavinder… - Theriogenology, 2019 - Elsevier
Preservation of cellular integrity and its mechanisms after ovarian tissue cryopreservation
(OTC) and in vitro culture (IVC) procedures are crucial aspects for the success of …

Intrafollicular injection of nanomolecules for advancing knowledge on folliculogenesis in livestock

JM Feugang, GM Ishak, MW Eggert, RD Arnold… - Theriogenology, 2022 - Elsevier
Despite the progress in assisted reproductive techniques, there is still a lack of rapid and
minimally invasive in situ approaches for further enhancements of female fertility. Therefore …