Bioassembly of complex iron–sulfur enzymes: hydrogenases and nitrogenases
Nature uses multinuclear metal clusters to catalyse a number of important multielectron
redox reactions. Examples that employ complex Fe–S clusters in catalysis include the Fe …
redox reactions. Examples that employ complex Fe–S clusters in catalysis include the Fe …
Biosynthesis of the catalytic H-cluster of [FeFe] hydrogenase: the roles of the Fe–S maturase proteins HydE, HydF, and HydG
[FeFe] hydrogenases carry out the redox interconversion of protons and molecular hydrogen
(2H++ 2e−⇌ H2) at a complex Fe–S active site known as the H-cluster. The H-cluster …
(2H++ 2e−⇌ H2) at a complex Fe–S active site known as the H-cluster. The H-cluster …
Mimicking the electron transport chain and active site of [FeFe] hydrogenases in one metal–organic framework: factors that influence charge transport
AT Castner, BA Johnson, SM Cohen… - Journal of the American …, 2021 - ACS Publications
[FeFe] hydrogenase (H2ase) enzymes are effective proton reduction catalysts capable of
forming molecular dihydrogen with a high turnover frequency at low overpotential. The …
forming molecular dihydrogen with a high turnover frequency at low overpotential. The …
Radical SAM enzyme HydE generates adenosylated Fe (I) intermediates en route to the [FeFe]-hydrogenase catalytic H-cluster
The H-cluster of [FeFe]-hydrogenase consists of a [4Fe–4S] H-subcluster linked by a
cysteinyl bridge to a unique organometallic [2Fe] H-subcluster assigned as the site of …
cysteinyl bridge to a unique organometallic [2Fe] H-subcluster assigned as the site of …
Fully Refined Semisynthesis of the [FeFe] Hydrogenase H-Cluster
[FeFe] hydrogenases contain a 6-Fe cofactor that serves as the active site for efficient redox
interconversion between H2 and protons. The biosynthesis of the so-called H-cluster …
interconversion between H2 and protons. The biosynthesis of the so-called H-cluster …
Stepwise isotope editing of [FeFe]-hydrogenases exposes cofactor dynamics
The six-iron cofactor of [FeFe]-hydrogenases (H-cluster) is the most efficient H2-forming
catalyst in nature. It comprises a diiron active site with three carbon monoxide (CO) and two …
catalyst in nature. It comprises a diiron active site with three carbon monoxide (CO) and two …
Evidence of Atypical Structural Flexibility of the Active Site Surrounding of an [FeFe] Hydrogenase from Clostridium beijerinkii
PS Corrigan, SH Majer, A Silakov - Journal of the American …, 2023 - ACS Publications
[FeFe] hydrogenase from Clostridium beijerinkii (Cb HydA1) is an unusual hydrogenase in
that it can withstand prolonged exposure to O2 by reversibly converting into an O2 …
that it can withstand prolonged exposure to O2 by reversibly converting into an O2 …
Organometallic Fe2(μ-SH)2(CO)4(CN)2 Cluster Allows the Biosynthesis of the [FeFe]-Hydrogenase with Only the HydF Maturase
The biosynthesis of the active site of the [FeFe]-hydrogenases (HydA1), the H-cluster, is of
interest because these enzymes are highly efficient catalysts for the oxidation and …
interest because these enzymes are highly efficient catalysts for the oxidation and …
A personal account on 25 years of scientific literature on [FeFe]-hydrogenase
JW Sidabras, ST Stripp - JBIC Journal of Biological Inorganic Chemistry, 2023 - Springer
Abstract [FeFe]-hydrogenases are gas-processing metalloenzymes that catalyze H2
oxidation and proton reduction (H2 release) in microorganisms. Their high turnover …
oxidation and proton reduction (H2 release) in microorganisms. Their high turnover …
From enzyme maturation to synthetic chemistry: the case of hydrogenases
Conspectus Water splitting into oxygen and hydrogen is one of the most attractive strategies
for storing solar energy and electricity. Because the processes at work are multielectronic …
for storing solar energy and electricity. Because the processes at work are multielectronic …