To overcome the radiation damage‐induced limitations to the resolution of three‐
dimensional reconstructions from electron microscopic tilt series, novel reconstruction …

Viruses are cellular parasites. The linkage between viral and host functions makes the study
of a viral life cycle an important key to cellular functions. A deeper understanding of many …

Recent advances in the field of electron cryomicroscopy (cryo-EM) have resulted in a rapidly
increasing number of atomic models of biomacromolecules that have been solved using this …

Structural flexibility and/or dynamic interactions with other molecules is a critical aspect of
protein function. Cryogenic electron microscopy (cryo-EM) provides direct visualization of …

Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

Resolution in optical nanoscopy (or super-resolution microscopy) depends on the
localization uncertainty and density of single fluorescent labels and on the sample's spatial …

A computational procedure is described for assigning the absolute hand of the structure of a
protein or assembly determined by single-particle electron microscopy. The procedure …

Earl J. Kirkland Third Edition Earl J. Kirkland Third Edition Page 1 Earl J. Kirkland Advanced
Computing in Electron Microscopy Third Edition Earl J. Kirkland Advanced Computing in …

The SPIDER system has evolved into a comprehensive tool set for image processing,
making use of modern graphics interfacing in the VMS and UNIX environment. SPIDER and …

The resolution value claimed for an electron microscopical three-dimensional reconstruction
indicates the overall quality of the experiment. The Fourier shell correlation (FSC) criterion …