Proteoforms are all forms of protein molecules from the same gene because of variations at
the DNA, RNA, and protein levels, eg, alternative splicing and post-translational …

Proteoforms, which arise from post-translational modifications, genetic polymorphisms and
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …

Mass spectrometry (MS)-based top-down proteomics (TDP) has revolutionized biological
research by measuring intact proteoforms in cells, tissues, and biofluids. Capillary zone …

We have performed CID experiments on a triple quadrupole instrument, lowering the
collision gas pressure by 50 times compared to its conventional value. The results show that …

Top–down proteomics (TDP) identifies, quantifies, and characterises proteins at the intact
proteoform level in complex biological samples to understand proteoform function and …

Quantitative analysis of proteins and their post-translational modifications (PTMs) in complex
biological samples is critical to understanding cellular biology as well as disease detection …

Abstract Background Cardiovascular diseases (CVDs) are the leading cause of death
worldwide, and the prevalence of CVDs increases markedly with age. Due to the high …

The quantification of proteoforms, ie, all molecular forms in which proteins can be present,
by top-down proteomics provides essential insights into biological processes at the …

Functional proteomics aims to elucidate biological functions, mechanisms, and pathways of
proteins and proteoforms at the molecular level to examine complex cellular systems and …

This Second Special Issue compiles 19 studies answering our open call to describe novel,
or at least significantly updated, Methods for Omics Research. The common thread among …