Carbohydrate-active enzymes (CAZymes) in the gut microbiome

JF Wardman, RK Bains, P Rahfeld… - Nature Reviews …, 2022 - nature.com
The 1013–1014 microorganisms present in the human gut (collectively known as the human
gut microbiota) dedicate substantial percentages of their genomes to the degradation and …

Reprint of: Advanced glycosidases as ingenious biosynthetic instruments

Z Mészáros, P Nekvasilová, P Bojarová, V Křen… - Biotechnology …, 2021 - Elsevier
Until recently, glycosidases, naturally hydrolyzing carbohydrate-active enzymes, have found
few synthetic applications in industry, being primarily used for cleaving unwanted …

Functional metagenomic screening identifies an unexpected β-glucuronidase

S Neun, P Brear, E Campbell, T Tryfona… - Nature Chemical …, 2022 - nature.com
The abundance of recorded protein sequence data stands in contrast to the small number of
experimentally verified functional annotation. Here we screened a million-membered …

Strategies for automated enzymatic glycan synthesis (AEGS)

KP Hussnaetter, P Palm, A Pich, M Franzreb… - Biotechnology …, 2023 - Elsevier
Glycans are the most abundant biopolymers on earth and are constituents of glycoproteins,
glycolipids, and proteoglycans with multiple biological functions. The availability of different …

Azido groups hamper glycan acceptance by carbohydrate processing enzymes

F Liu, HM Chen, Z Armstrong, SG Withers - ACS central science, 2022 - ACS Publications
Azido sugars have found frequent use as probes of biological systems in approaches
ranging from cell surface metabolic labeling to activity-based proteomic profiling of …

Discovery and development of promiscuous O-glycan hydrolases for removal of intact sialyl T-antigen

JF Wardman, P Rahfeld, F Liu… - ACS Chemical …, 2021 - ACS Publications
Mucin-type O-glycosylation (O-glycosylation) is a common post-translational modification
that confers distinct biophysical properties to proteins and plays crucial roles in intercellular …

Emerging enzyme surface display systems for waste resource recovery

B Davenport, SJ Hallam - Environmental Microbiology, 2023 - Wiley Online Library
The current century marks an inflection point for human progress, as the developed world
increasingly comes to recognize that the ecological and socioeconomic impacts of resource …

Mining microbial resources from water

YN Liu, S Deng, S Bairoliya, B Cao - Resources, Conservation and …, 2023 - Elsevier
In the past two decades, the concept of microbial resources evolved from the conserved
strains in culture collection centers and microbial genome data in databases to functional …

Characterization of an Unknown Region Linked to the Glycoside Hydrolase Family 17 β-1,3-Glucanase of Vibrio vulnificus Reveals a Novel Glucan-Binding Domain

Y Kumagai, H Kishimura, W Lang, T Tagami… - Marine drugs, 2022 - mdpi.com
The glycoside hydrolase family 17 β-1, 3-glucanase of Vibrio vulnificus (VvGH17) has two
unknown regions in the N-and C-termini. Here, we characterized these domains by …

Metagenomic profiling of rhizosphere microbiota: Unraveling the plant-soil dynamics

AK Wani, F Rahayu, AM Alkahtani, MA Alreshidi… - … and Molecular Plant …, 2024 - Elsevier
Rhizosphere is a dynamic and complex environment shaped by intricate interactions
between plants and a diverse microbial community. The rhizosphere microbiome is crucial …