Intracellular delivery is a key step in biological research and has enabled decades of
biomedical discoveries. It is also becoming increasingly important in industrial and medical …

The CRISPR/Cas9 system is a rapid, simple, and often extremely efficient gene editing
method. This method has been used in a variety of organisms and cell types over the past …

Translation of the CRISPR–Cas9 system to human therapeutics holds high promise.
However, specificity remains a concern especially when modifying stem cell populations …

Genomics has greatly improved how patients with cancer are being treated; however,
clinical-grade genomic biomarkers for chemotherapies are currently lacking. Using whole …

Background Conditional knockout mice and transgenic mice expressing recombinases,
reporters, and inducible transcriptional activators are key for many genetic studies and …

Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits
relatively poor editing efficiency, restricting its overall utility. Here we isolate an engineered …

CRISPR/Cas9-based genome editing can easily generate knockout mouse models by
disrupting the gene sequence, but its efficiency for creating models that require either …

CRISPR systems have emerged as transformative tools for altering genomes in living cells
with unprecedented ease, inspiring keen interest in increasing their specificity for perfectly …

Protease-activated receptor 4 (PAR4)(gene F2RL3) harbors a functional dimorphism,
rs773902 A/G (encoding Thr120/Ala120, respectively) and is associated with greater platelet …

Inconsistent activity limits the use of CRISPR-Cas9 in zebrafish. We show supernumerary
guanine nucleotides at the 5′ ends of single guide RNAs (sgRNAs) account for diminished …