The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of
recombinant proteins, frequently outperforming other heterologous hosts. Already, a …

The ends of chromosomal DNA double-strand breaks (DSBs) can be accurately rejoined by
at least two discrete pathways, homologous recombination and nonhomologous end-joining …

The BRCA1 gene encodes a tumor suppressor that is mutated in 50% of familial breast
cancers. The BRCA1 protein has been implicated in the DNA damage response, as DNA …

Abstract The Saccharomyces cerevisiae Rad53 protein kinase is required for the execution
of checkpoint arrest at multiple stages of the cell cycle. We found that Rad53 …

Inherited and germ-line de novo copy number variants (CNVs) are increasingly found to be
correlated with human developmental and cancerous phenotypes. Several models for …

Bifunctional alkylating agents and other drugs which produce DNA interstrand cross-links
(ICLs) are among the most effective antitumor agents in clinical use. In contrast to agents …

Homologous recombination (HR) contributes to maintaining genome integrity by facilitating
error-free repair of DNA double-strand breaks (DSBs) primarily during the S and G2 phases …

Genotoxic agents can cause replication fork stalling in dividing cells because of DNA
lesions, eventually leading to replication fork collapse when the damage is not repaired …

Gene targeting is a challenge in organisms where non-homologous end-joining is the
predominant form of recombination. We show that cell division cycle synchronization can be …

The DNA damage response (DDR) is an evolutionarily conserved process essential for cell
survival. The transcription changes triggered by DDR depend on the nature of DNA damage …