Intracytoplasmic sperm injection for male infertility and consequences for offspring

SC Esteves, M Roque, G Bedoschi, T Haahr… - Nature Reviews …, 2018 - nature.com
Intracytoplasmic sperm injection (ICSI) has become the most commonly used method of
fertilization in assisted reproductive technology. The primary reasons for its popularity stem …

Sperm oxidative stress during in vitro manipulation and its effects on sperm function and embryo development

R Gualtieri, G Kalthur, V Barbato, S Longobardi… - Antioxidants, 2021 - mdpi.com
Reactive oxygen species (ROS) generated at low levels during mitochondrial respiration
have key roles in several signaling pathways. Oxidative stress (OS) arises when the …

Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples

MM Quinn, L Jalalian, S Ribeiro, K Ona… - Human …, 2018 - academic.oup.com
STUDY QUESTION Does microfluidic sorting improve the selection of sperm with lower DNA
fragmentation over standard density-gradient centrifugation? SUMMARY ANSWER …

Sperm quality metrics were improved by a biomimetic microfluidic selection platform compared to swim-up methods

SA Vasilescu, L Ding, FY Parast, R Nosrati… - Microsystems & …, 2023 - nature.com
Sperm selection is an essential component of all assisted reproductive treatments (ARTs)
and is by far the most neglected step in the ART workflow in regard to technological …

[HTML][HTML] Separation efficiency of a microfluidic sperm sorter to minimize sperm DNA damage

K Shirota, F Yotsumoto, H Itoh, H Obama, N Hidaka… - Fertility and sterility, 2016 - Elsevier
Objective To evaluate whether microfluidic sperm sorters (MFSSs) allow effective recovery of
sorted motile sperm without DNA damage compared with the centrifugation and swim-up …

Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample

H Jeon, C Cremers, D Le, J Abell, J Han - Scientific reports, 2022 - nature.com
Here, we propose a fully-automated platform using a spiral inertial microfluidic device for
standardized semen preparation that can process patient-derived semen samples with …

Magnetotactic sperm cells for assisted reproduction

F Striggow, C Ribeiro, A Aziz, R Nauber, F Hebenstreit… - Small, 2024 - Wiley Online Library
Biohybrid micromotors are active microscopic agents consisting of biological and synthetic
components that are being developed as novel tools for biomedical applications. By …

Sperm selection strategies and their impact on assisted reproductive technology outcomes

S Pinto, DF Carrageta, MG Alves, A Rocha… - Andrologia, 2021 - Wiley Online Library
The application of assisted reproductive technologies (ART) has revolutionised the
treatment of human infertility, giving hope to the patients previously considered incapable of …

The impact of sperm DNA fragmentation on ICSI outcome in cases of donated oocytes

S Antonouli, A Papatheodorou, Y Panagiotidis… - Archives of gynecology …, 2019 - Springer
Purpose The aim of this study is to evaluate the sperm DNA fragmentation index (DFI) in
oocyte donation cycles and correlate it with the sperm parameters, the male characteristics …

[HTML][HTML] Selecting the most competent sperm for assisted reproductive technologies

RS Jeyendran, E Caroppo, A Rouen, A Anderson… - Fertility and …, 2019 - Elsevier
This paper discusses the variety of effective sperm selection techniques that have been
developed for use in assisted reproductive technologies. Available methods for isolating the …