The importance of safer approaches for gene therapy has been underscored by a series of
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …

Different approaches (including association studies) have recently been adopted for the
functional characterization of allelic variation in plants and to identify sequence motifs …

Agricultural biotechnology is limited by the inefficiencies of conventional random
mutagenesis and transgenesis. Because targeted genome modification in plants has been …

The efficient repair of double-strand breaks (DSBs) in genomic DNA is important for the
survival of all organisms. In recent years, basic mechanisms of DSB repair in somatic plant …

Targeted mutagenesis is an essential tool of reverse genetics that could be used
experimentally to investigate basic plant biology or modify crop plants for improvement of …

Homologous recombination offers great promise for plant genome engineering. This
promise has not been realized, however, because when DNA enters plant cells homologous …

Genetic transformation mediated by Agrobacterium involves the transfer of a DNA molecule
(T-DNA) from the bacterium to the eukaryotic host cell, and its integration into the host …

The Ecological Society of America has evaluated the ecological effects of current and
potential uses of field‐released genetically engineered organisms (GEOs), as described in …

Only five years after the initiation of transgenic research in plants, gene targeting (GT) was
achieved for the first time in tobacco. Unfortunately, the frequency of targeted integration via …

Modification of genes through homologous recombination, termed gene targeting, is the
most direct method to characterize gene function. In higher plants, however, the method is …