An N-degron is a degradation signal whose main determinant is a “destabilizing” N-terminal
residue of a protein. Specific N-degrons, discovered in 1986, were the first identified …

Cellular functions are mediated by protein–protein interactions, and mapping the
interactome provides fundamental insights into biological systems. Affinity purification …

A diverse antibody repertoire is essential for humoral immunity. Antibody diversification
requires the introduction of deoxyuridine (dU) mutations within immunoglobulin genes to …

Specificity of the ubiquitin-proteasome system depends on E3 ligase-substrate interactions.
Many such pairings depend on E3 ligases binding to peptide-like sequences-termed N-or C …

Mammalian target of rapamycin (mTOR) senses changes in nutrient status and stimulates
the autophagic process to recycle amino acids. However, the impact of nutrient stress on …

Ubiquitin ligases (E3s) are pivotal specificity determinants in the ubiquitin system by
selecting substrates and decorating them with distinct ubiquitin signals. However, structure …

Papain-like cysteine peptidases form a big and highly diverse superfamily of proteins
involved in many important biological functions, such as protein turnover, deubiquitination …

Almost all mitochondrial proteins are encoded by nuclear genes and synthesized in the
cytosol as precursor proteins. Signals in the amino acid sequence of these precursors …

The yeast glucose-induced degradation-deficient (GID) E3 ubiquitin ligase forms a suite of
complexes with interchangeable receptors that selectively recruit N-terminal degron motifs of …

The human CTLH/GID (hGID) complex emerged as an important E3 ligase regulating
multiple cellular processes, including cell cycle progression and metabolism. However, the …