Surface-initiated controlled radical polymerization: state-of-the-art, opportunities, and challenges in surface and interface engineering with polymer brushes

JO Zoppe, NC Ataman, P Mocny, J Wang… - Chemical …, 2017 - ACS Publications
The generation of polymer brushes by surface-initiated controlled radical polymerization (SI-
CRP) techniques has become a powerful approach to tailor the chemical and physical …

Poly (N-isopropylacrylamide)-based thermoresponsive surfaces provide new types of biomedical applications

K Nagase, M Yamato, H Kanazawa, T Okano - Biomaterials, 2018 - Elsevier
Thermoresponsive surfaces, prepared by grafting of poly (N-isopropylacrylamide)(PIPAAm)
or its copolymers, have been investigated for biomedical applications. Thermoresponsive …

Thermoresponsive-polymer-based materials for temperature-modulated bioanalysis and bioseparations

K Nagase, T Okano - Journal of Materials Chemistry B, 2016 - pubs.rsc.org
Many bioactive compounds, pharmaceutical proteins, and therapeutic cells are used in
medical treatments. Methods for the effective purification of such compounds that retain their …

Thermoresponsive block copolymer brush for temperature-modulated hepatocyte separation

K Nagase, N Kojima, M Goto, T Akaike… - Journal of Materials …, 2022 - pubs.rsc.org
Hepatic tissue engineering may be an effective approach for the treatment of liver disease;
however, its practical application requires hepatic cell separation technologies that do not …

Antibody drug separation using thermoresponsive anionic polymer brush modified beads with optimised electrostatic and hydrophobic interactions

K Nagase, S Ishii, K Ikeda, S Yamada, D Ichikawa… - Scientific reports, 2020 - nature.com
Antibody drugs play an important role in biopharmaceuticals, because of the specificity for
target biomolecules and reduction of side effects. Thus, separation and analysis techniques …

Thermally-modulated cell separation columns using a thermoresponsive block copolymer brush as a packing material for the purification of mesenchymal stem cells

K Nagase, G Edatsune, Y Nagata, J Matsuda… - Biomaterials …, 2021 - pubs.rsc.org
Cell therapy using mesenchymal stem cells (MSCs) is used as effective regenerative
treatment. Cell therapy requires effective cell separation without cell modification and …

Temperature-modulated antibody drug separation using thermoresponsive mixed polymer brush-modified stationary phase

K Nagase, S Ishii, A Takeuchi, H Kanazawa - Separation and Purification …, 2022 - Elsevier
Recently, antibody drugs have been investigated for medical treatments of intractable
diseases. An effective antibody drug separation method is in demand for production of such …

Poly (N-isopropylacrylamide) based thermoresponsive polymer brushes for bioseparation, cellular tissue fabrication, and nano actuators

K Nagase, T Okano, H Kanazawa - Nano-Structures & Nano-Objects, 2018 - Elsevier
Many thermoresponsive surfaces for biomedical applications have been developed using
various techniques for modifying substrates with thermoresponsive polymers. In addition …

Protein purification using solid-phase extraction on temperature-responsive hydrogel-modified silica beads

K Okubo, K Ikeda, A Oaku, Y Hiruta, K Nagase… - … of Chromatography A, 2018 - Elsevier
Recently, the importance of biopharmaceuticals in medical treatments has been increasing,
and effective protein purification methods are strongly required for their production. In the …

Dynamically cell separating thermo-functional biointerfaces with densely packed polymer brushes

K Nagase, A Kimura, T Shimizu, K Matsuura… - Journal of Materials …, 2012 - pubs.rsc.org
Poly (N-isopropylacrylamide)(PIPAAm) brush grafted glass surfaces with various brush
lengths were prepared as cell separating intelligent interfaces through a surface-initiated …