The application of single-chain Fv fragments (scFv) in medicine and biotechnology places
great demands on their stability. Only recently has attention been given to the production of …

▪ Abstract Antibodies are unique in their high affinity and specificity for a binding partner, a
quality that has made them one of the most useful molecules for biotechnology and …

Conventional methods for humanizing animal-derived antibodies involve grafting their
complementarity-determining regions onto homologous human framework regions …

There are great demands on the stability, expression yield and resistance to aggregation of
antibody fragments. To untangle intrinsic domain effects from domain interactions, we …

To increase the valency, stability and therapeutic potential of bispecific antibodies, we
designed a novel recombinant molecule that is bispecific and tetravalent. It was constructed …

TIGIT (T-cell immunoglobulin and ITIM domain) has emerged as a promising target in cancer
immunotherapy. It is an immune “checkpoint” inhibitor primarily expressed on activated T …

Engineering T cells with chimeric antigen receptors (CARs) is an effective method for
directing T cells to attack tumors, but may cause adverse side effects such as the potentially …

Rapid production of protein-based tumor-specific vaccines for the treatment of malignancies
is possible with the plant-based transient expression system described here. We created a …

A library of mutants of a single-chain Fv fragment (scFv) was generated by a combination of
directed and random mutagenesis, using oligonucleotides randomized at defined positions …

For treatment of patients with prostate cancer (PCa), we developed a novel T cell-engaging
(BiTE) antibody designated AMG 212 or BAY2010112 that is bispecific for prostate-specific …