Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …

Synthetic gene networks have wide-ranging uses in reprogramming and rewiring
organisms. To date, there has not been a way to harness the vast potential of these networks …

We report on and provide a detailed characterization of the performance and properties of a
recently developed, all Escherichia coli, cell-free transcription and translation system. Gene …

Genetic circuits and reaction cascades are of great importance for synthetic biology,
biochemistry and bioengineering. An open question is how to maximize the modularity of …

There is a growing need to enhance our capabilities in medical and environmental
diagnostics. Synthetic biologists have begun to focus their biomolecular engineering …

Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization …

Cell-free protein synthesis has emerged as a powerful approach for expanding the range of
genetically encoded chemistry into proteins. Unfortunately, efforts to site-specifically …

Native cell-free transcription–translation systems offer a rapid route to characterize the
regulatory elements (promoters, transcription factors) for gene expression from nonmodel …