Hairy root research: recent scenario and exciting prospects

S Guillon, J Tremouillaux-Guiller, PK Pati… - Current opinion in plant …, 2006 - Elsevier
High stability of the production of secondary metabolites is an interesting characteristic of
hairy root cultures. For 25 years, hairy roots have been investigated as a biological system …

Engineering secondary metabolite production in hairy roots

S Chandra, R Chandra - Phytochemistry reviews, 2011 - Springer
Plants are a rich source of valuable secondary metabolites. Plant cell culture technologies
developed in the past as possible tools for secondary metabolites production. In most cases …

Phaseolus vulgaris lectins: A systematic review of characteristics and health implications

S He, BK Simpson, H Sun, MO Ngadi… - Critical reviews in food …, 2018 - Taylor & Francis
Legume lectins are carbohydrate-binding proteins of non-immune origin. Significant
amounts of lectins have been found in Phaseolus vulgaris beans as far back as in the last …

An overview of lectins purification strategies

KS Nascimento, AI Cunha… - Journal of Molecular …, 2012 - Wiley Online Library
Lectins hold great promise not only as reagents for diagnostics and drug discovery but also
as a novel class of biopharmaceutical products. In fact, new research directions in the last …

Expression and purification of pharmaceutical proteins in plants

Q Chen - Biological Engineering Transactions, 2008 - elibrary.asabe.org
The majority of human pharmaceuticals including vaccine and therapeutic proteins are
currently produced in mammalian and microbial cell cultures. However, these technology …

Improving containment strategies in biopharming

DJ Murphy - Plant Biotechnology Journal, 2007 - Wiley Online Library
This review examines the challenges of segregating biopharmed crops expressing
pharmaceutical or veterinary agents from mainstream crops, particularly those destined for …

Production of mouse interleukin‐12 is greater in tobacco hairy roots grown in a mist reactor than in an airlift reactor

C Liu, MJ Towler, G Medrano… - Biotechnology and …, 2009 - Wiley Online Library
We compared the growth and productivity of a tobacco line of hairy roots that produces
murine interleukin 12 (mIL‐12) grown in three different culture systems: shake flasks, an …

The partitioning of protease from Calotropis procera latex by aqueous two-phase systems and its hydrolytic pattern on muscle proteins

S Rawdkuen, P Pintathong, P Chaiwut… - Food and Bioproducts …, 2011 - Elsevier
The protease from the latex of Calotropis procera was isolated by an aqueous two-phase
system (ATPS). Polyethylene glycol (PEG 1000, 2000 and 3000) at a concentration of 12 …

A capture and release method based on noncovalent ligand cross-linking and facile filtration for purification of lectins and glycoproteins

CJ Welch, ML Talaga, PD Kadav, JL Edwards… - Journal of Biological …, 2020 - ASBMB
Glycan-binding proteins such as lectins are ubiquitous proteins that mediate many biological
functions. To study their various biological activities and structure–function relationships …

Biomass production of hairy roots of Artemisia annua and Arachis hypogaea in a scaled‐up mist bioreactor

G Sivakumar, C Liu, MJ Towler… - Biotechnology and …, 2010 - Wiley Online Library
Hairy roots have the potential to produce a variety of valuable small and large molecules.
The mist reactor is a gas phase bioreactor that has shown promise for low‐cost culture of …