Single molecule force spectroscopy is a powerful method that uses the mechanical
properties of DNA to explore DNA interactions. Here we describe how DNA stretching …

DNA intercalators are widely used as fluorescent probes to visualize DNA and DNA
transactions in vivo and in vitro. It is well known that they perturb DNA structure and stability …

The last decade has seen rapid development in single molecule manipulation of RNA and
DNA. Measuring the response force for a particular manipulation has allowed the free …

Single-molecule manipulation studies have revealed that double-stranded DNA undergoes
a structural transition when subjected to tension. At forces that depend on the attachment …

SYBR Gold is a commonly used and particularly bright fluorescent DNA stain, however, its
chemical structure is unknown and its binding mode to DNA remains controversial. Here, we …

There is a wide range of applications for non-covalent DNA binding ligands, and
optimization of such interactions requires detailed understanding of the binding …

We used single DNA molecule stretching to investigate DNA intercalation by ethidium and
three ruthenium complexes. By measuring ligand-induced DNA elongation at different …

DNA-binding small molecules are widespread in the cell and heavily used in biological
applications. Here, we use magnetic tweezers, which control the force and torque applied to …

Here we explore DNA binding by a family of ruthenium (II) polypyridyl complexes using an
atomic force microscope (AFM) and optical tweezers. We demonstrate using AFM that Ru …

The HIV-1 nucleocapsid protein (NC) is a multi-functional protein necessary for viral
replication. Recent studies have demonstrated reverse transcription occurs inside the fully …