Advances and challenges in biotechnological production of chondroitin sulfate and its oligosaccharides

W Zhang, R Xu, J Chen, H Xiong, Y Wang… - International journal of …, 2023 - Elsevier
Chondroitin sulfate (CS) is a member of glycosaminoglycans (GAGs) and has critical
physiological functions. CS is widely applied in medical and clinical fields. Currently, the …

Human milk oligosaccharides (HMOS): structure, function, and enzyme-catalyzed synthesis

X Chen - Advances in carbohydrate chemistry and biochemistry, 2015 - Elsevier
The important roles played by human milk oligosaccharides (HMOS), the third major
component of human milk, in the health of breast-fed infants have been increasingly …

Biotechnological advances in UDP-sugar based glycosylation of small molecules

F De Bruyn, J Maertens, J Beauprez, W Soetaert… - Biotechnology …, 2015 - Elsevier
Glycosylation of small molecules like specialized (secondary) metabolites has a profound
impact on their solubility, stability or bioactivity, making glycosides attractive compounds as …

Spatial co-localization of multi-enzymes by inorganic nanocrystal–protein complexes

Z Li, Y Zhang, Y Su, P Ouyang, J Ge… - Chemical Communications, 2014 - pubs.rsc.org
We report a simple precipitation method for the construction of spatially co-localized multi-
enzyme systems based on inorganic nanocrystal–protein complexes. A spatially controlled …

Efficient chemoenzymatic synthesis of an N-glycan isomer library

L Li, Y Liu, C Ma, J Qu, AD Calderon, B Wu, N Wei… - Chemical …, 2015 - pubs.rsc.org
Quantification, characterization and biofunctional studies of N-glycans on proteins remain
challenging tasks due to the complexity, diversity and low abundance of these glycans. The …

One-pot multienzyme (OPME) systems for chemoenzymatic synthesis of carbohydrates

H Yu, X Chen - Organic & biomolecular chemistry, 2016 - pubs.rsc.org
Glycosyltransferase-catalyzed enzymatic and chemoenzymatic syntheses are powerful
approaches for the production of oligosaccharides, polysaccharides, glycoconjugates, and …

Tools for studying glycans: recent advances in chemoenzymatic glycan labeling

A Lopez Aguilar, JG Briard, L Yang, B Ovryn… - ACS chemical …, 2017 - ACS Publications
The study of cellular glycosylation presents many challenges due, in large part, to the
nontemplated nature of glycan biosynthesis and glycans' structural complexity …

Effective sugar nucleotide regeneration for the large-scale enzymatic synthesis of Globo H and SSEA4

TI Tsai, HY Lee, SH Chang, CH Wang… - Journal of the …, 2013 - ACS Publications
We report here the development of chemoenzymatic methods for the large-scale synthesis
of cancer-associated antigens globopentaose (Gb5), fucosyl-Gb5 (Globo H), and sialyl-Gb5 …

A Bacterial β1–3-Galactosyltransferase Enables Multigram-Scale Synthesis of Human Milk Lacto-N-tetraose (LNT) and Its Fucosides

JB McArthur, H Yu, X Chen - ACS catalysis, 2019 - ACS Publications
β1–3-Linked galactosides such as Galβ1–3GlcNAcβOR are common carbohydrate motifs
found in human milk oligosaccharides (HMOSs), glycolipids, and glycoproteins. Efficient and …

Current state on the enzymatic synthesis of glycosaminoglycans

J Gottschalk, L Elling - Current opinion in chemical biology, 2021 - Elsevier
Glycosaminoglycans (GAGs) are linear anionic polysaccharides, and most of them show a
specific sulfation pattern. GAGs have been studied for decades, and still, new biological …