Detecting and characterizing circular RNAs

WR Jeck, NE Sharpless - Nature biotechnology, 2014 - nature.com
Circular RNA transcripts were first identified in the early 1990s but knowledge of these
species has remained limited, as their study through traditional methods of RNA analysis …

RNA circularization strategies in vivo and in vitro

S Petkovic, S Müller - Nucleic acids research, 2015 - academic.oup.com
In the plenitude of naturally occurring RNAs, circular RNAs (circRNAs) and their biological
role were underestimated for years. However, circRNAs are ubiquitous in all domains of life …

[HTML][HTML] A review of polymers as multifunctional excipients in drug dosage form technology

B Karolewicz - Saudi Pharmaceutical Journal, 2016 - Elsevier
In the article, groups of multifunctional polymers used in drug dosage form technology were
classified and evaluated. These compounds, in addition to their basic function as excipients …

Characterizing and circumventing sequence restrictions for synthesis of circular RNA in vitro

JW Rausch, WF Heinz, MJ Payea… - Nucleic Acids …, 2021 - academic.oup.com
Just as eukaryotic circular RNA (circRNA) is a product of intracellular backsplicing, custom
circRNA can be synthesized in vitro using a transcription template in which transposed …

Enzymatic polymerization of cyclic monomers in ionic liquids as a prospective synthesis method for polyesters used in drug delivery systems

U Piotrowska, M Sobczak - Molecules, 2014 - mdpi.com
Biodegradable or bioresorbable polymers are commonly used in various pharmaceutical
fields (eg, as drug delivery systems, therapeutic systems or macromolecular drug …

Autodisplay of an endo-1, 4-β-xylanase from Clostridium cellulovorans in Escherichia coli for xylans degradation

VEB Hernández, CJ Salas-Montantes… - Enzyme and microbial …, 2021 - Elsevier
The goal of this work was the autodisplay of the endo β-1, 4-xylanase (XynA) from
Clostridium cellulovorans in Escherichia coli using the AIDA system to carry out whole-cell …

Charge profiling and stability testing of biosimilar by capillary isoelectric focusing

J Cao, W Sun, F Gong, W Liu - Electrophoresis, 2014 - Wiley Online Library
CIEF was developed for the rapid analysis of charge heterogeneity of trastuzumab biosimilar
using commercially available fluorocarbon‐coated capillary. The CIEF master mix was …

Segmented polyurethane elastomers derived from aliphatic polycarbonate and poly (ester‐carbonate) soft segments for biomedical applications

M Sobczak, C Dębek, E Olędzka… - Journal of Polymer …, 2012 - Wiley Online Library
A series of biomedical polyurethane elastomers (PURs) based on poly (ester‐carbonate) s
(PECs) and polycarbonates (PCs) were synthesized and spectrally characterized fully. PEC …

Improvement of in vitro bulblet formation in garlic under continuous-light condition is tightly associated with FLOWERING LOCUS T-like gene expression

K Tadamura, A Torada - Plant Cell, Tissue and Organ Culture (PCTOC), 2023 - Springer
Garlic (Allium sativum L.) in vitro bulblet formation is elicited by low temperatures and
subsequent long photoperiods. FLOWERING LOCUS T (FT)-like genes control photoperiod …

[HTML][HTML] A Simple and Efficient One-Step Synthesis System for Flexible Production of Circular RNA in E. coli

X Zhao, Y Liu, H Huang, Y Sun, F Wu, W Jin - Biomolecules, 2024 - mdpi.com
Circular RNA (circRNA) exhibits a higher stability and intracellular half-life than linear RNA
and has better potential in the fields of RNA vaccines and RNAi drugs. The current strategies …