Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

We give a methodology-oriented perspective on directional image analysis and rotation-
invariant processing. We review the state of the art in the field and make connections with …

Methods based on single-molecule localization and photophysics have brought nanoscale
imaging with visible light into reach. This has enabled single-particle tracking applications …

We optimally localized isolated fluorescent beads and molecules imaged as diffraction-
limited spots, determined the orientation of molecules and present reliable formulas for the …

Super-resolution localization microscopy methods provide powerful new capabilities for
probing biology at the nanometer scale via fluorescence. These methods rely on two key …

Numerous endocytic accessory proteins (EAPs) mediate assembly and maturation of
clathrin-coated pits (CCPs) into cargo-containing vesicles. Analysis of EAP function through …

Estimation of a parameter of interest from image data represents a task that is commonly
carried out in single molecule microscopy data analysis. The determination of the positional …

We present a reconstruction algorithm that resolves cellular tractions in diffraction-limited
nascent adhesions (NAs). The enabling method is the introduction of sparsity regularization …

Recently, single molecule-based superresolution fluorescence microscopy has surpassed
the diffraction limit to improve resolution to the order of 20 nm or better. These methods …

Essential cellular functions as diverse as genome maintenance and tissue morphogenesis
rely on the dynamic organization of filamentous assemblies. For example, the precise …