[HTML][HTML] Enhanced CRISPR/Cas12a-based quantitative detection of nucleic acids using double emulsion droplets
Y Zhang, H Liu, Y Nakagawa, Y Nagasaka… - Biosensors and …, 2024 - Elsevier
Pairing droplet microfluidics and CRISPR/Cas12a techniques creates a powerful solution for
the detection and quantification of nucleic acids at the single-molecule level, due to its …
the detection and quantification of nucleic acids at the single-molecule level, due to its …
[HTML][HTML] Design automation of microfluidic single and double emulsion droplets with machine learning
A Lashkaripour, DP McIntyre, SGK Calhoun… - Nature …, 2024 - nature.com
Droplet microfluidics enables kHz screening of picoliter samples at a fraction of the cost of
other high-throughput approaches. However, generating stable droplets with desired …
other high-throughput approaches. However, generating stable droplets with desired …
DEPICT-seq: Single-Cell Transcriptomic Analysis of Rare Cell Subsets Isolated via Nucleic Acid Cytometry
The ability to dive deep into specific rare cell populations is critical for understanding tissue
physiology and pathology across various biological domains. As single-cell RNA-seq …
physiology and pathology across various biological domains. As single-cell RNA-seq …
High‐Throughput Single‐Cell, Single‐Mitochondrial DNA Assay Using Hydrogel Droplet Microfluidics
There is growing interest in understanding the biological implications of single cell
heterogeneity and heteroplasmy of mitochondrial DNA (mtDNA), but current methodologies …
heterogeneity and heteroplasmy of mitochondrial DNA (mtDNA), but current methodologies …
High‐precision screening and sorting of double emulsion droplets
Mounting evidence suggests that cell populations are extremely heterogeneous, with
individual cells fulfilling different roles within the population. Flow cytometry (FC) is a high …
individual cells fulfilling different roles within the population. Flow cytometry (FC) is a high …