The lack of safe and efficient gene-delivery methods is a limiting obstacle to human gene
therapy. Synthetic gene-delivery agents, although safer than recombinant viruses, generally …

The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in
biotechnology. Toward this end, rapid advances have recently been made in our …

Encapsulation of drugs within nanocarriers that selectively target malignant cells promises to
mitigate side effects of conventional chemotherapy and to enable delivery of the unique drug …

For many years, it was generally accepted that mRNA is too unstable to be efficiently used
for gene therapy purposes. In the last decade, however, several research groups faced this …

Many therapeutics require efficient cytosolic delivery either because the receptors for those
drugs are located in the cytosol or their site of action is an intracellular organelle that …

The last 10 years have seen substantial progress in the development and application of
nonviral vectors in gene therapy. However, many problems remain to be resolved before …

The CRISPR-Cas9 targeted nuclease technology allows the insertion of genetic
modifications with single base-pair precision. The preference of mammalian cells to repair …

The improvement of non‐viral‐based gene delivery systems is of prime importance for the
future of gene and antisense therapies. We have previously described a peptide‐based …

The long cherished goal of targeting drugs to specific sites in the body, where the
pharmacological action is desired and sparing other tissues has been actively pursued all …

Cationic lipid/DNA lipoplexes and cationic polymer/DNA polyplexes represent an attractive
alternative to viral vectors for cell transfection in vitro and in vivo but still suffer from a …