The independence of research—a review of disciplinary perspectives and outline of interdisciplinary prospects

J Gläser, M Ash, G Buenstorf, D Hopf, L Hubenschmid… - Minerva, 2022 - Springer
The independence of research is a key strategic issue of modern societies. Dealing with it
appropriately poses legal, economic, political, social and cultural problems for society, which …

Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications

S Mital, G Christie, D Dikicioglu - Microbial cell factories, 2021 - Springer
Recombinant enzyme expression in Escherichia coli is one of the most popular methods to
produce bulk concentrations of protein product. However, this method is often limited by the …

Integration of Genetic and Process Engineering for Optimized Rhamnolipid Production Using Pseudomonas putida

T Tiso, N Ihling, S Kubicki, A Biselli… - … in bioengineering and …, 2020 - frontiersin.org
Rhamnolipids are biosurfactants produced by microorganisms with the potential to replace
synthetic compounds with petrochemical origin. To promote industrial use of rhamnolipids …

Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures

M Mühlmann, E Forsten, S Noack, J Büchs - Microbial Cell Factories, 2017 - Springer
Background Escherichia coli (E. coli) is the most abundant expression host for recombinant
proteins. The production efficiency is dependent on a multitude of parameters. Therefore …

Hydrogen online monitoring based on thermal conductivity for anaerobic microorganisms

K Miebach, M Finger, AMK Scherer… - Biotechnology and …, 2023 - Wiley Online Library
H2‐producing microorganisms are a promising source of sustainable biohydrogen.
However, most H2‐producing microorganisms are anaerobes, which are difficult to cultivate …

Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank …

SJ Wewetzer, M Kunze, T Ladner… - Journal of Biological …, 2015 - Springer
Background Conventional experiments in small scale are often performed in a 'Black
Box'fashion, analyzing only the product concentration in the final sample. Online monitoring …

Device for respiration activity measurement enables the determination of oxygen transfer rates of microbial cultures in shaken 96‐deepwell microtiter plates

R Dinger, C Lattermann, D Flitsch… - Biotechnology and …, 2022 - Wiley Online Library
Mini‐bioreactors with integrated online monitoring capabilities are well established in the
early stages of process development. Mini‐bioreactors fulfil the demand for high‐throughput …

Respiration activity monitoring system for any individual well of a 48-well microtiter plate

D Flitsch, S Krabbe, T Ladner, M Beckers… - Journal of biological …, 2016 - Springer
Background Small-scale micro-bioreactors have become the cultivation vessel of choice
during the first steps of bioprocess development. They combine high cultivation throughput …

Maximizing the stability of metabolic engineering‐derived whole‐cell biocatalysts

M Kadisch, C Willrodt, M Hillen, B Bühler… - Biotechnology …, 2017 - Wiley Online Library
A systematic and powerful knowledge‐based framework exists for improving the activity and
stability of chemical catalysts and for empowering the commercialization of respective …

A synthetic reaction cascade implemented by colocalization of two proteins within catalytically active inclusion bodies

VD Jäger, R Lamm, R Kloß, E Kaganovitch… - ACS synthetic …, 2018 - ACS Publications
In nature, enzymatic reaction cascades, ie, realized in metabolic networks, operate with
unprecedented efficacy, with the reactions often being spatially and temporally orchestrated …