Droplet microfluidics for microbiology: techniques, applications and challenges

TS Kaminski, O Scheler, P Garstecki - Lab on a Chip, 2016 - pubs.rsc.org
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

Concentration gradient generation methods based on microfluidic systems

X Wang, Z Liu, Y Pang - RSC advances, 2017 - pubs.rsc.org
Various concentration gradient generation methods based on microfluidic systems are
summarized in this paper. The review covers typical structural characteristics, gradient …

Droplet microfluidics for high-throughput analysis of antibiotic susceptibility in bacterial cells and populations

W Postek, P Garstecki - Accounts of Chemical Research, 2022 - ACS Publications
Conspectus Antibiotic-resistant bacteria are an increasing concern both in everyday life and
specialized environments such as healthcare. As the rate of antibiotic-resistant infections …

Suzuki–Miyaura cross-coupling optimization enabled by automated feedback

BJ Reizman, YM Wang, SL Buchwald… - Reaction chemistry & …, 2016 - pubs.rsc.org
An automated, droplet-flow microfluidic system explores and optimizes Pd-catalyzed Suzuki–
Miyaura cross-coupling reactions. A smart optimal DoE-based algorithm is implemented to …

Individual control and quantification of 3D spheroids in a high-density microfluidic droplet array

RFX Tomasi, S Sart, T Champetier, CN Baroud - Cell reports, 2020 - cell.com
As three-dimensional cell culture formats gain in popularity, there emerges a need for tools
that produce vast amounts of data on individual cells within the spheroids or organoids …

Accelerating bacterial growth detection and antimicrobial susceptibility assessment in integrated picoliter droplet platform

AM Kaushik, K Hsieh, L Chen, DJ Shin, JC Liao… - Biosensors and …, 2017 - Elsevier
There remains an urgent need for rapid diagnostic methods that can evaluate antibiotic
resistance for pathogenic bacteria in order to deliver targeted antibiotic treatments. Toward …

Microfluidic on-demand droplet merging using surface acoustic waves

M Sesen, T Alan, A Neild - Lab on a Chip, 2014 - pubs.rsc.org
Individual droplets can be isolated within microfluidic systems by use of an immiscible
carrier layer. This type of two phase systems, often termed “digital microfluidics”, find wide …

Bacterial growth and adaptation in microdroplet chemostats

S Jakiela, TS Kaminski, O Cybulski… - Angewandte …, 2013 - Wiley Online Library
We describe herein microfluidic technology for manipulating and monitoring continuous
growth of populations of bacteria. A system consisting of approximately ten input and output …

Droplet-based microfluidics: enabling impact on drug discovery

OJ Dressler, RM Maceiczyk… - Journal of …, 2014 - journals.sagepub.com
Over the past two decades, the application of microengineered systems in the chemical and
biological sciences has transformed the way in which high-throughput experimentation is …

Droplet electrospray ionization mass spectrometry for high throughput screening for enzyme inhibitors

S Sun, RT Kennedy - Analytical chemistry, 2014 - ACS Publications
High throughput screening (HTS) is important for identifying molecules with desired
properties. Mass spectrometry (MS) is potentially powerful for label-free HTS due to its high …