From bulk, single-cell to spatial RNA sequencing
X Li, CY Wang - International journal of oral science, 2021 - nature.com
RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in
addition to differential gene expression, thus providing a more complete genetic picture than …
addition to differential gene expression, thus providing a more complete genetic picture than …
Stem cell fate in cancer growth, progression and therapy resistance
Although we have come a long way in our understanding of the signals that drive cancer
growth, and how these signals can be targeted, effective control of this disease remains a …
growth, and how these signals can be targeted, effective control of this disease remains a …
SpaceM reveals metabolic states of single cells
A growing appreciation of the importance of cellular metabolism and revelations concerning
the extent of cell–cell heterogeneity demand metabolic characterization of individual cells …
the extent of cell–cell heterogeneity demand metabolic characterization of individual cells …
Gene expression based inference of cancer drug sensitivity
S Chawla, A Rockstroh, M Lehman, E Ratther… - Nature …, 2022 - nature.com
Inter and intra-tumoral heterogeneity are major stumbling blocks in the treatment of cancer
and are responsible for imparting differential drug responses in cancer patients. Recently …
and are responsible for imparting differential drug responses in cancer patients. Recently …
Massively parallel digital transcriptional profiling of single cells
Characterizing the transcriptome of individual cells is fundamental to understanding
complex biological systems. We describe a droplet-based system that enables 3′ mRNA …
complex biological systems. We describe a droplet-based system that enables 3′ mRNA …
[HTML][HTML] Loss of SYNCRIP unleashes APOBEC-driven mutagenesis, tumor heterogeneity, and AR-targeted therapy resistance in prostate cancer
Tumor mutational burden and heterogeneity has been suggested to fuel resistance to many
targeted therapies. The cytosine deaminase APOBEC proteins have been implicated in the …
targeted therapies. The cytosine deaminase APOBEC proteins have been implicated in the …
stLearn: integrating spatial location, tissue morphology and gene expression to find cell types, cell-cell interactions and spatial trajectories within undissociated tissues
Spatial Transcriptomics is an emerging technology that adds spatial dimensionality and
tissue morphology to the genome-wide transcriptional profile of cells in an undissociated …
tissue morphology to the genome-wide transcriptional profile of cells in an undissociated …
High-throughput methods in the discovery and study of biomaterials and materiobiology
The complex interaction of cells with biomaterials (ie, materiobiology) plays an increasingly
pivotal role in the development of novel implants, biomedical devices, and tissue …
pivotal role in the development of novel implants, biomedical devices, and tissue …
Design and computational analysis of single-cell RNA-sequencing experiments
R Bacher, C Kendziorski - Genome biology, 2016 - Springer
Single-cell RNA-sequencing (scRNA-seq) has emerged as a revolutionary tool that allows
us to address scientific questions that eluded examination just a few years ago. With the …
us to address scientific questions that eluded examination just a few years ago. With the …
A general approach for detecting expressed mutations in AML cells using single cell RNA-sequencing
Virtually all tumors are genetically heterogeneous, containing mutationally-defined
subclonal cell populations that often have distinct phenotypes. Single-cell RNA-sequencing …
subclonal cell populations that often have distinct phenotypes. Single-cell RNA-sequencing …