Nanodiscs in membrane biochemistry and biophysics

IG Denisov, SG Sligar - Chemical reviews, 2017 - ACS Publications
Membrane proteins play a most important part in metabolism, signaling, cell motility,
transport, development, and many other biochemical and biophysical processes which …

Design and use of model membranes to study biomolecular interactions using complementary surface-sensitive techniques

LA Clifton, RA Campbell, F Sebastiani… - Advances in Colloid and …, 2020 - Elsevier
Cellular membranes are complex structures and simplified analogues in the form of model
membranes or biomembranes are used as platforms to understand fundamental properties …

Polymers and surfactants at fluid interfaces studied with specular neutron reflectometry

L Braun, M Uhlig, R von Klitzing… - Advances in colloid and …, 2017 - Elsevier
This review addresses the advances made with specular neutron reflectometry in studies of
aqueous mixtures of polymers and surfactants at fluid interfaces during the last decade (or …

Polyelectrolyte/surfactant films spread from neutral aggregates

RA Campbell, A Tummino, BA Noskov, I Varga - Soft Matter, 2016 - pubs.rsc.org
We describe a new methodology to prepare loaded polyelectrolyte/surfactant films at the
air/water interface by exploiting Marangoni spreading resulting from the dynamic …

Characterization of lipid bilayers adsorbed to functionalized air/water interfaces

J Pusterla, E Scoppola, C Appel, T Mukhina, C Shen… - Nanoscale, 2022 - pubs.rsc.org
Lipid bilayers immobilized in planar geometries, such as solid-supported or “floating”
bilayers, have enabled detailed studies of biological membranes with numerous …

Evidence of lipid exchange in styrene maleic acid lipid particle (SMALP) nanodisc systems

G Hazell, T Arnold, RD Barker, LA Clifton, NJ Steinke… - Langmuir, 2016 - ACS Publications
Styrene-alt-maleic acid lipid particles (SMALPs) are self-assembled discoidal structures
composed of a polymer belt and a segment of lipid bilayer, which are capable of …

Monitoring shifts in the conformation equilibrium of the membrane protein cytochrome P450 reductase (POR) in nanodiscs

M Wadsäter, T Laursen, A Singha, NS Hatzakis… - Journal of Biological …, 2012 - ASBMB
Nanodiscs are self-assembled∼ 50-nm 2 patches of lipid bilayers stabilized by amphipathic
belt proteins. We demonstrate that a well ordered dense film of nanodiscs serves for non …

[HTML][HTML] Suspended phospholipid bilayers: A new biological membrane mimetic

SE Ayscough, LA Clifton, MWA Skoda… - Journal of Colloid and …, 2023 - Elsevier
Hypothesis The attractive interaction between a cationic surfactant monolayer at the air–
water interface and vesicles, incorporating anionic lipids, is sufficient to drive the adsorption …

Human lipoproteins at model cell membranes: effect of lipoprotein class on lipid exchange

KL Browning, TK Lind, S Maric… - Scientific Reports, 2017 - nature.com
High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset
and development of atherosclerosis; the biggest killer in the western world. Key issues of …

Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli

S Maric, MB Thygesen, J Schiller, M Marek… - Applied microbiology …, 2015 - Springer
Phosphatidylcholine (PC) is a major component of eukaryotic cell membranes and one of
the most commonly used phospholipids for reconstitution of membrane proteins into carrier …