Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels

JB Grimm, LD Lavis - Nature Methods, 2022 - nature.com
The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

[HTML][HTML] The interplay between BAX and BAK tunes apoptotic pore growth to control mitochondrial-DNA-mediated inflammation

K Cosentino, V Hertlein, A Jenner, T Dellmann… - Molecular cell, 2022 - cell.com
BAX and BAK are key apoptosis regulators that mediate the decisive step of mitochondrial
outer membrane permeabilization. However, the mechanism by which they assemble the …

MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells

KC Gwosch, JK Pape, F Balzarotti, P Hoess… - Nature …, 2020 - nature.com
The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …

Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope

R Schmidt, T Weihs, CA Wurm, I Jansen… - Nature …, 2021 - nature.com
The recently introduced minimal photon fluxes (MINFLUX) concept pushed the resolution of
fluorescence microscopy to molecular dimensions. Initial demonstrations relied on custom …

Dna-paint minflux nanoscopy

LM Ostersehlt, DC Jans, A Wittek, J Keller-Findeisen… - Nature …, 2022 - nature.com
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient
fluorophore localizations, has brought about three-dimensional resolution at nanometer …

Deep learning enables fast and dense single-molecule localization with high accuracy

A Speiser, LR Müller, P Hoess, U Matti, CJ Obara… - Nature …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) has had remarkable success in imaging
cellular structures with nanometer resolution, but standard analysis algorithms require …

Direct observation of motor protein stepping in living cells using MINFLUX

T Deguchi, MK Iwanski, EM Schentarra, C Heidebrecht… - Science, 2023 - science.org
Dynamic measurements of molecular machines can provide invaluable insights into their
mechanism, but these measurements have been challenging in living cells. Here, we …

Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging

S Fu, W Shi, T Luo, Y He, L Zhou, J Yang, Z Yang… - Nature …, 2023 - nature.com
Single-molecule localization microscopy in a typical wide-field setup has been widely used
for investigating subcellular structures with super resolution; however, field-dependent …