Computational tools and resources for CRISPR/Cas genome editing
The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
Design and analysis of CRISPR–Cas experiments
A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
Potent and uniform fetal hemoglobin induction via base editing
Inducing fetal hemoglobin (HbF) in red blood cells can alleviate β-thalassemia and sickle
cell disease. We compared five strategies in CD34+ hematopoietic stem and progenitor …
cell disease. We compared five strategies in CD34+ hematopoietic stem and progenitor …
Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
Eukaryotic genomes contain domesticated genes from integrating viruses and mobile
genetic elements. Among these are homologs of the capsid protein (known as Gag) of long …
genetic elements. Among these are homologs of the capsid protein (known as Gag) of long …
Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity
B Prinzing, CC Zebley, CT Petersen, Y Fan… - Science translational …, 2021 - science.org
Chimeric antigen receptor (CAR) T cell therapy is revolutionizing cancer immunotherapy for
patients with B cell malignancies and is now being developed for solid tumors and chronic …
patients with B cell malignancies and is now being developed for solid tumors and chronic …
Base editing of haematopoietic stem cells rescues sickle cell disease in mice
Sickle cell disease (SCD) is caused by a mutation in the β-globin gene HBB. We used a
custom adenine base editor (ABE8e-NRCH), to convert the SCD allele (HBB S) into …
custom adenine base editor (ABE8e-NRCH), to convert the SCD allele (HBB S) into …
Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …
However, the currently most practicable approaches rely on the generation of DNA double …
cBAF complex components and MYC cooperate early in CD8+ T cell fate
The identification of mechanisms to promote memory T (Tmem) cells has important
implications for vaccination and anti-cancer immunotherapy,,–. Using a CRISPR-based …
implications for vaccination and anti-cancer immunotherapy,,–. Using a CRISPR-based …
Base-resolution models of transcription-factor binding reveal soft motif syntax
Ž Avsec, M Weilert, A Shrikumar, S Krueger… - Nature …, 2021 - nature.com
The arrangement (syntax) of transcription factor (TF) binding motifs is an important part of the
cis-regulatory code, yet remains elusive. We introduce a deep learning model, BPNet, that …
cis-regulatory code, yet remains elusive. We introduce a deep learning model, BPNet, that …
[HTML][HTML] Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer
CD8+ cytotoxic T cells (CTLs) orchestrate antitumour immunity and exhibit inherent
heterogeneity,, with precursor exhausted T (Tpex) cells but not terminally exhausted T (Tex) …
heterogeneity,, with precursor exhausted T (Tpex) cells but not terminally exhausted T (Tex) …