Advances and challenges in small‐molecule DNA aptamer isolation, characterization, and sensor development

H Yu, O Alkhamis, J Canoura, Y Liu… - Angewandte Chemie …, 2021 - Wiley Online Library
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind
to specific molecules with high affinity, and offer a number of advantages relative to …

Isothermal titration calorimetry

M Bastos, O Abian, CM Johnson… - Nature Reviews …, 2023 - nature.com
Isothermal titration calorimetry (ITC) has become the gold standard for studying molecular
interactions in solution. Although it is increasingly being used in the soft matter and synthetic …

The antibiotic darobactin mimics a β-strand to inhibit outer membrane insertase

H Kaur, RP Jakob, JK Marzinek, R Green, Y Imai… - Nature, 2021 - nature.com
Antibiotics that target Gram-negative bacteria in new ways are needed to resolve the
antimicrobial resistance crisis,–. Gram-negative bacteria are protected by an additional outer …

Bioengineering a plant NLR immune receptor with a robust binding interface toward a conserved fungal pathogen effector

R Zdrzałek, Y Xi, T Langner, AR Bentham… - Proceedings of the …, 2024 - pnas.org
Bioengineering of plant immune receptors has emerged as a key strategy for generating
novel disease resistance traits to counteract the expanding threat of plant pathogens to …

A blast fungus zinc-finger fold effector binds to a hydrophobic pocket in host Exo70 proteins to modulate immune recognition in rice

JC De la Concepcion, K Fujisaki… - Proceedings of the …, 2022 - National Acad Sciences
Exocytosis plays an important role in plant–microbe interactions, in both pathogenesis and
symbiosis. Exo70 proteins are integral components of the exocyst, an octameric complex …

Perception of structurally distinct effectors by the integrated WRKY domain of a plant immune receptor

N Mukhi, H Brown, D Gorenkin… - Proceedings of the …, 2021 - National Acad Sciences
Plants use intracellular nucleotide-binding domain (NBD) and leucine-rich repeat (LRR)–
containing immune receptors (NLRs) to detect pathogen-derived effector proteins. The …

Enzyme kinetics by isothermal titration calorimetry: Allostery, inhibition, and dynamics

Y Wang, G Wang, N Moitessier… - Frontiers in molecular …, 2020 - frontiersin.org
Isothermal titration calorimetry (ITC) involves accurately measuring the heat that is released
or absorbed in real time when one solution is titrated into another. This technique is usually …

Lewis acidity scale of diaryliodonium ions toward oxygen, nitrogen, and halogen lewis bases

RJ Mayer, AR Ofial, H Mayr… - Journal of the American …, 2020 - ACS Publications
Equilibrium constants for the associations of 17 diaryliodonium salts Ar2I+ X–with 11
different Lewis bases (halide ions, carboxylates, p-nitrophenolate, amines, and tris (p-anisyl) …

Mapping the gaps in chemical analysis for the characterisation of aptamer-target interactions

E Daems, G Moro, R Campos, K De Wael - TrAC Trends in Analytical …, 2021 - Elsevier
Aptamers are promising biorecognition elements with a wide applicability from therapeutics
to biosensing. However, to successfully use these biomolecules, a complete …

Lewis acidic boranes, Lewis bases, and equilibrium constants: a reliable scaffold for a quantitative Lewis acidity/basicity scale

RJ Mayer, N Hampel, AR Ofial - Chemistry–A European …, 2021 - Wiley Online Library
A quantitative Lewis acidity/basicity scale toward boron‐centered Lewis acids has been
developed based on a set of 90 experimental equilibrium constants for the reactions of …